CRISPR-U™ Gene Knockout Cell Line Strategy

KDM1A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human KDM1A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol KDM1A
Gene id 23028
Organism Homo sapiens
Official full symbol lysine demethylase 1A
Gene type protein-coding
Also known as AOF2, BHC110, CPRF, KDM1, LSD1
Summary This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 1
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
KDM1A-202 ENST00000400181.9 3085 876aa Protein coding CCDS53278 O60341-2 NM_001009999.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
KDM1A-203 ENST00000465864.2 3039 858aa Protein coding CCDS85939 R4GMQ1 - TSL:5, GENCODE basic,
KDM1A-201 ENST00000356634.7 3033 852aa Protein coding CCDS30627 O60341-1 - TSL:1, GENCODE basic,
KDM1A-205 ENST00000494920.1 905 301aa Protein coding - R4GMP9 - CDS 5' and 3' incomplete, TSL:3,
KDM1A-206 ENST00000602503.1 2810 No protein Retained intron - - - TSL:NA,
KDM1A-204 ENST00000481879.1 525 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene KDM1A
This KO Strategy loading
Red Cotton™ Notes Gene KDM1A had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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