CRISPR-U™ Gene Knockout Cell Line
Strategy
LIMCH1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LIMCH1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LIMCH1 |
| Gene id | 22998 |
| Organism | Homo sapiens |
| Official full symbol | LIM and calponin homology domains 1 |
| Gene type | protein-coding |
| Also known as | LIMCH1A, LMO7B |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 17 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LIMCH1-205 | ENST00000503057.6 | 7697 | 1467aa | Protein coding | CCDS82919 | G5EA03 | NM_001330672.2 | TSL:1, GENCODE basic, MANE Select v0.92, |
| LIMCH1-201 | ENST00000313860.11 | 6165 | 1083aa | Protein coding | CCDS33977 | Q9UPQ0-1 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| LIMCH1-203 | ENST00000396595.7 | 5740 | 902aa | Protein coding | CCDS47047 | Q9UPQ0-6 | - | TSL:1, GENCODE basic, |
| LIMCH1-202 | ENST00000381753.8 | 5705 | 890aa | Protein coding | CCDS54765 | Q9UPQ0-9 | - | TSL:1, GENCODE basic, |
| LIMCH1-218 | ENST00000513024.5 | 5129 | 910aa | Protein coding | CCDS75121 | Q9UPQ0-5 | - | TSL:1, GENCODE basic, |
| LIMCH1-216 | ENST00000512820.5 | 5071 | 1069aa | Protein coding | CCDS75119 | Q9UPQ0-4 | - | TSL:1, GENCODE basic, |
| LIMCH1-217 | ENST00000512946.5 | 5057 | 1057aa | Protein coding | CCDS54763 | Q9UPQ0-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| LIMCH1-208 | ENST00000508501.5 | 5054 | 1056aa | Protein coding | CCDS54764 | Q9UPQ0-10 | - | TSL:1, GENCODE basic, APPRIS P4, |
| LIMCH1-215 | ENST00000512632.5 | 4792 | 980aa | Protein coding | CCDS87221 | D6RD46 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| LIMCH1-209 | ENST00000509277.5 | 3474 | 916aa | Protein coding | CCDS82920 | E9PDJ9 | - | TSL:2, GENCODE basic, |
| LIMCH1-213 | ENST00000511496.5 | 3439 | 897aa | Protein coding | CCDS87222 | Q9UPQ0-8 | - | TSL:1, GENCODE basic, |
| LIMCH1-219 | ENST00000514096.5 | 3543 | 897aa | Protein coding | - | E7EPK0 | - | TSL:1, GENCODE basic, |
| LIMCH1-207 | ENST00000508466.1 | 3051 | 917aa | Protein coding | - | H0Y8P3 | - | CDS 5' incomplete, TSL:2, |
| LIMCH1-211 | ENST00000509638.5 | 1570 | 153aa | Protein coding | - | D6RJ93 | - | TSL:1, GENCODE basic, |
| LIMCH1-210 | ENST00000509454.5 | 1506 | 160aa | Protein coding | - | Q6NVB9 | - | TSL:1, GENCODE basic, |
| LIMCH1-204 | ENST00000446625.6 | 789 | 126aa | Protein coding | - | D6R8Y0 | - | CDS 3' incomplete, TSL:5, |
| LIMCH1-212 | ENST00000511424.5 | 551 | 26aa | Protein coding | - | D6RGH8 | - | CDS 3' incomplete, TSL:3, |
| LIMCH1-221 | ENST00000515785.1 | 1129 | No protein | Processed transcript | - | - | - | TSL:1, |
| LIMCH1-220 | ENST00000514190.5 | 540 | No protein | Processed transcript | - | - | - | TSL:4, |
| LIMCH1-222 | ENST00000515847.1 | 490 | No protein | Processed transcript | - | - | - | TSL:4, |
| LIMCH1-214 | ENST00000512228.5 | 473 | No protein | Processed transcript | - | - | - | TSL:4, |
| LIMCH1-206 | ENST00000505705.1 | 437 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LIMCH1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LIMCH1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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