CRISPR-U™ Gene Knockout Cell Line Strategy

LIMCH1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LIMCH1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LIMCH1
Gene id 22998
Organism Homo sapiens
Official full symbol LIM and calponin homology domains 1
Gene type protein-coding
Also known as LIMCH1A, LMO7B
Genomic regions Chromosome 4
Strategy Summary
This gene has 17 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LIMCH1-205 ENST00000503057.6 7697 1467aa Protein coding CCDS82919 G5EA03 NM_001330672.2 TSL:1, GENCODE basic, MANE Select v0.92,
LIMCH1-201 ENST00000313860.11 6165 1083aa Protein coding CCDS33977 Q9UPQ0-1 - TSL:1, GENCODE basic, APPRIS ALT2,
LIMCH1-203 ENST00000396595.7 5740 902aa Protein coding CCDS47047 Q9UPQ0-6 - TSL:1, GENCODE basic,
LIMCH1-202 ENST00000381753.8 5705 890aa Protein coding CCDS54765 Q9UPQ0-9 - TSL:1, GENCODE basic,
LIMCH1-218 ENST00000513024.5 5129 910aa Protein coding CCDS75121 Q9UPQ0-5 - TSL:1, GENCODE basic,
LIMCH1-216 ENST00000512820.5 5071 1069aa Protein coding CCDS75119 Q9UPQ0-4 - TSL:1, GENCODE basic,
LIMCH1-217 ENST00000512946.5 5057 1057aa Protein coding CCDS54763 Q9UPQ0-2 - TSL:1, GENCODE basic, APPRIS ALT2,
LIMCH1-208 ENST00000508501.5 5054 1056aa Protein coding CCDS54764 Q9UPQ0-10 - TSL:1, GENCODE basic, APPRIS P4,
LIMCH1-215 ENST00000512632.5 4792 980aa Protein coding CCDS87221 D6RD46 - TSL:1, GENCODE basic, APPRIS ALT2,
LIMCH1-209 ENST00000509277.5 3474 916aa Protein coding CCDS82920 E9PDJ9 - TSL:2, GENCODE basic,
LIMCH1-213 ENST00000511496.5 3439 897aa Protein coding CCDS87222 Q9UPQ0-8 - TSL:1, GENCODE basic,
LIMCH1-219 ENST00000514096.5 3543 897aa Protein coding - E7EPK0 - TSL:1, GENCODE basic,
LIMCH1-207 ENST00000508466.1 3051 917aa Protein coding - H0Y8P3 - CDS 5' incomplete, TSL:2,
LIMCH1-211 ENST00000509638.5 1570 153aa Protein coding - D6RJ93 - TSL:1, GENCODE basic,
LIMCH1-210 ENST00000509454.5 1506 160aa Protein coding - Q6NVB9 - TSL:1, GENCODE basic,
LIMCH1-204 ENST00000446625.6 789 126aa Protein coding - D6R8Y0 - CDS 3' incomplete, TSL:5,
LIMCH1-212 ENST00000511424.5 551 26aa Protein coding - D6RGH8 - CDS 3' incomplete, TSL:3,
LIMCH1-221 ENST00000515785.1 1129 No protein Processed transcript - - - TSL:1,
LIMCH1-220 ENST00000514190.5 540 No protein Processed transcript - - - TSL:4,
LIMCH1-222 ENST00000515847.1 490 No protein Processed transcript - - - TSL:4,
LIMCH1-214 ENST00000512228.5 473 No protein Processed transcript - - - TSL:4,
LIMCH1-206 ENST00000505705.1 437 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LIMCH1
This KO Strategy loading
Red Cotton™ Notes Gene LIMCH1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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