CRISPR-U™ Gene Knockout Cell Line Strategy

PTGR1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTGR1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTGR1
Gene id 22949
Organism Homo sapiens
Official full symbol prostaglandin reductase 1
Gene type protein-coding
Also known as LTB4DH, PGR1, ZADH3
Summary This gene encodes an enzyme that is involved in the inactivation of the chemotactic factor, leukotriene B4. The encoded protein specifically catalyzes the NADP+ dependent conversion of leukotriene B4 to 12-oxo-leukotriene B4. A pseudogene of this gene is found on chromosome 1. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 9
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTGR1-205 ENST00000407693.7 1599 329aa Protein coding CCDS6779 Q14914-1 NM_001146108.2 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PTGR1-201 ENST00000309195.9 1223 329aa Protein coding CCDS6779 Q14914-1 - TSL:1, GENCODE basic, APPRIS P1,
PTGR1-209 ENST00000538962.7 1200 301aa Protein coding CCDS55331 Q14914-2 - TSL:2, GENCODE basic,
PTGR1-206 ENST00000422125.1 461 119aa Protein coding - Q5JVP2 - CDS 3' incomplete, TSL:3,
PTGR1-204 ENST00000374324.5 2298 73aa Nonsense mediated decay - F6XGT7 - TSL:5,
PTGR1-207 ENST00000466771.6 1941 300aa Nonsense mediated decay - F2Z3J9 - TSL:3,
PTGR1-203 ENST00000374313.5 1061 No protein Retained intron - - - TSL:1,
PTGR1-202 ENST00000374308.1 847 No protein Retained intron - - - TSL:2,
PTGR1-208 ENST00000485319.1 389 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTGR1
This KO Strategy loading
Red Cotton™ Notes Gene PTGR1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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$49

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Work flow
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