CRISPR-U™ Gene Knockout Cell Line Strategy

DKK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DKK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DKK1
Gene id 22943
Organism Homo sapiens
Official full symbol dickkopf WNT signaling pathway inhibitor 1
Gene type protein-coding
Also known as DKK-1, SK
Summary This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines.
Genomic regions Chromosome 10
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DKK1-201 ENST00000373970.4 1805 266aa Protein coding CCDS7246 I1W660 O94907 NM_012242.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DKK1-202 ENST00000467359.5 547 No protein Processed transcript - - - TSL:2,
DKK1-203 ENST00000476752.1 544 No protein Processed transcript - - - TSL:2,
DKK1-204 ENST00000494277.5 465 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DKK1
This KO Strategy loading
Red Cotton™ Notes Gene DKK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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