CRISPR-U™ Gene Knockout Cell Line
Strategy
DKK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DKK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DKK1 |
| Gene id | 22943 |
| Organism | Homo sapiens |
| Official full symbol | dickkopf WNT signaling pathway inhibitor 1 |
| Gene type | protein-coding |
| Also known as | DKK-1, SK |
| Summary | This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DKK1-201 | ENST00000373970.4 | 1805 | 266aa | Protein coding | CCDS7246 | I1W660 O94907 | NM_012242.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DKK1-202 | ENST00000467359.5 | 547 | No protein | Processed transcript | - | - | - | TSL:2, |
| DKK1-203 | ENST00000476752.1 | 544 | No protein | Processed transcript | - | - | - | TSL:2, |
| DKK1-204 | ENST00000494277.5 | 465 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DKK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DKK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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