CRISPR-U™ Gene Knockout Cell Line
Strategy
ELL2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ELL2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ELL2 |
| Gene id | 22936 |
| Organism | Homo sapiens |
| Official full symbol | elongation factor for RNA polymerase II 2 |
| Gene type | protein-coding |
| Also known as | MRCCAT1 |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ELL2-201 | ENST00000237853.9 | 5826 | 640aa | Protein coding | CCDS4080 | O00472-1 | NM_012081.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ELL2-206 | ENST00000513343.1 | 582 | 151aa | Protein coding | - | D6RC27 | - | CDS 3' incomplete, TSL:3, |
| ELL2-205 | ENST00000508757.1 | 468 | 137aa | Protein coding | - | H0Y8G1 | - | CDS 5' incomplete, TSL:4, |
| ELL2-208 | ENST00000635633.1 | 467 | 65aa | Nonsense mediated decay | - | A0A0U1RR08 | - | CDS 5' incomplete, TSL:3, |
| ELL2-203 | ENST00000506628.1 | 520 | No protein | Processed transcript | - | - | - | TSL:5, |
| ELL2-204 | ENST00000508694.1 | 743 | No protein | Retained intron | - | - | - | TSL:5, |
| ELL2-202 | ENST00000505584.1 | 730 | No protein | Retained intron | - | - | - | TSL:2, |
| ELL2-207 | ENST00000515020.1 | 340 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ELL2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ELL2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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