CRISPR-U™ Gene Knockout Cell Line Strategy

ELL2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ELL2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ELL2
Gene id 22936
Organism Homo sapiens
Official full symbol elongation factor for RNA polymerase II 2
Gene type protein-coding
Also known as MRCCAT1
Genomic regions Chromosome 5
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ELL2-201 ENST00000237853.9 5826 640aa Protein coding CCDS4080 O00472-1 NM_012081.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ELL2-206 ENST00000513343.1 582 151aa Protein coding - D6RC27 - CDS 3' incomplete, TSL:3,
ELL2-205 ENST00000508757.1 468 137aa Protein coding - H0Y8G1 - CDS 5' incomplete, TSL:4,
ELL2-208 ENST00000635633.1 467 65aa Nonsense mediated decay - A0A0U1RR08 - CDS 5' incomplete, TSL:3,
ELL2-203 ENST00000506628.1 520 No protein Processed transcript - - - TSL:5,
ELL2-204 ENST00000508694.1 743 No protein Retained intron - - - TSL:5,
ELL2-202 ENST00000505584.1 730 No protein Retained intron - - - TSL:2,
ELL2-207 ENST00000515020.1 340 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ELL2
This KO Strategy loading
Red Cotton™ Notes Gene ELL2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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