CRISPR-U™ Gene Knockout Cell Line Strategy

ATF6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ATF6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ATF6
Gene id 22926
Organism Homo sapiens
Official full symbol activating transcription factor 6
Gene type protein-coding
Also known as ACHM7, ATF6A
Summary This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis.
Genomic regions Chromosome 1
Strategy Summary
This gene has 11 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ATF6-201 ENST00000367942.4 7470 670aa Protein coding CCDS1235 P18850 NM_007348.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
ATF6-204 ENST00000679853.1 5509 669aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ATF6-203 ENST00000679833.1 4367 577aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ATF6-212 ENST00000681036.1 3178 604aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ATF6-210 ENST00000680688.1 3111 689aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ATF6-216 ENST00000681541.1 2996 604aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ATF6-207 ENST00000680462.1 2974 609aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ATF6-221 ENST00000681912.1 2757 542aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ATF6-215 ENST00000681492.1 2487 700aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ATF6-205 ENST00000679886.1 2426 468aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ATF6-209 ENST00000680633.1 611 150aa Protein coding - - - CDS 3' incomplete,
ATF6-218 ENST00000681738.1 3768 670aa Nonsense mediated decay CCDS1235 - - -
ATF6-220 ENST00000681801.1 2307 670aa Nonsense mediated decay CCDS1235 - - -
ATF6-211 ENST00000681001.1 3084 57aa Nonsense mediated decay - - - -
ATF6-217 ENST00000681557.1 3032 64aa Nonsense mediated decay - - - -
ATF6-213 ENST00000681169.1 2811 286aa Nonsense mediated decay - - - -
ATF6-208 ENST00000680481.1 2233 89aa Nonsense mediated decay - - - -
ATF6-206 ENST00000680180.1 3135 No protein Processed transcript - - - -
ATF6-219 ENST00000681779.1 2000 No protein Processed transcript - - - -
ATF6-214 ENST00000681187.1 1934 No protein Processed transcript - - - -
ATF6-202 ENST00000476437.1 778 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ATF6
This KO Strategy loading
Red Cotton™ Notes Gene ATF6 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×