CRISPR-U™ Gene Knockout Cell Line
Strategy
ATF6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ATF6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ATF6 |
| Gene id | 22926 |
| Organism | Homo sapiens |
| Official full symbol | activating transcription factor 6 |
| Gene type | protein-coding |
| Also known as | ACHM7, ATF6A |
| Summary | This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ATF6-201 | ENST00000367942.4 | 7470 | 670aa | Protein coding | CCDS1235 | P18850 | NM_007348.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| ATF6-204 | ENST00000679853.1 | 5509 | 669aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ATF6-203 | ENST00000679833.1 | 4367 | 577aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ATF6-212 | ENST00000681036.1 | 3178 | 604aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ATF6-210 | ENST00000680688.1 | 3111 | 689aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ATF6-216 | ENST00000681541.1 | 2996 | 604aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ATF6-207 | ENST00000680462.1 | 2974 | 609aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ATF6-221 | ENST00000681912.1 | 2757 | 542aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ATF6-215 | ENST00000681492.1 | 2487 | 700aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ATF6-205 | ENST00000679886.1 | 2426 | 468aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ATF6-209 | ENST00000680633.1 | 611 | 150aa | Protein coding | - | - | - | CDS 3' incomplete, |
| ATF6-218 | ENST00000681738.1 | 3768 | 670aa | Nonsense mediated decay | CCDS1235 | - | - | - |
| ATF6-220 | ENST00000681801.1 | 2307 | 670aa | Nonsense mediated decay | CCDS1235 | - | - | - |
| ATF6-211 | ENST00000681001.1 | 3084 | 57aa | Nonsense mediated decay | - | - | - | - |
| ATF6-217 | ENST00000681557.1 | 3032 | 64aa | Nonsense mediated decay | - | - | - | - |
| ATF6-213 | ENST00000681169.1 | 2811 | 286aa | Nonsense mediated decay | - | - | - | - |
| ATF6-208 | ENST00000680481.1 | 2233 | 89aa | Nonsense mediated decay | - | - | - | - |
| ATF6-206 | ENST00000680180.1 | 3135 | No protein | Processed transcript | - | - | - | - |
| ATF6-219 | ENST00000681779.1 | 2000 | No protein | Processed transcript | - | - | - | - |
| ATF6-214 | ENST00000681187.1 | 1934 | No protein | Processed transcript | - | - | - | - |
| ATF6-202 | ENST00000476437.1 | 778 | No protein | Processed transcript | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ATF6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ATF6 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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