CRISPR-U™ Gene Knockout Cell Line Strategy

MMRN1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MMRN1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MMRN1
Gene id 22915
Organism Homo sapiens
Official full symbol multimerin 1
Gene type protein-coding
Also known as ECM, EMILIN4, GPIa*, MMRN
Summary Multimerin is a massive, soluble protein found in platelets and in the endothelium of blood vessels. It is comprised of subunits linked by interchain disulfide bonds to form large, variably sized homomultimers. Multimerin is a factor V/Va-binding protein and may function as a carrier protein for platelet factor V. It may also have functions as an extracellular matrix or adhesive protein. Recently, patients with an unusual autosomal-dominant bleeding disorder (factor V Quebec) were found to have a deficiency of platelet multimerin.
Genomic regions Chromosome 4
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MMRN1-202 ENST00000394980.5 5217 1228aa Protein coding CCDS3635 Q13201-1 - TSL:5, GENCODE basic, APPRIS P1,
MMRN1-201 ENST00000264790.7 5001 1228aa Protein coding CCDS3635 Q13201-1 NM_007351.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MMRN1-204 ENST00000508372.1 3234 970aa Protein coding - E7EPG1 - TSL:2, GENCODE basic,
MMRN1-203 ENST00000506328.1 766 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MMRN1
This KO Strategy loading
Red Cotton™ Notes Gene MMRN1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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