CRISPR-U™ Gene Knockout Cell Line
Strategy
MMRN1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MMRN1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MMRN1 |
| Gene id | 22915 |
| Organism | Homo sapiens |
| Official full symbol | multimerin 1 |
| Gene type | protein-coding |
| Also known as | ECM, EMILIN4, GPIa*, MMRN |
| Summary | Multimerin is a massive, soluble protein found in platelets and in the endothelium of blood vessels. It is comprised of subunits linked by interchain disulfide bonds to form large, variably sized homomultimers. Multimerin is a factor V/Va-binding protein and may function as a carrier protein for platelet factor V. It may also have functions as an extracellular matrix or adhesive protein. Recently, patients with an unusual autosomal-dominant bleeding disorder (factor V Quebec) were found to have a deficiency of platelet multimerin. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MMRN1-202 | ENST00000394980.5 | 5217 | 1228aa | Protein coding | CCDS3635 | Q13201-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| MMRN1-201 | ENST00000264790.7 | 5001 | 1228aa | Protein coding | CCDS3635 | Q13201-1 | NM_007351.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MMRN1-204 | ENST00000508372.1 | 3234 | 970aa | Protein coding | - | E7EPG1 | - | TSL:2, GENCODE basic, |
| MMRN1-203 | ENST00000506328.1 | 766 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MMRN1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MMRN1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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