CRISPR-U™ Gene Knockout Cell Line Strategy

PPP6R1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPP6R1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PPP6R1
Gene id 22870
Organism Homo sapiens
Official full symbol protein phosphatase 6 regulatory subunit 1
Gene type protein-coding
Also known as KIAA1115, PP6R1, SAP190, SAPS1
Summary Protein phosphatase regulatory subunits, such as SAPS1, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. SAPS1 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C; MIM 612725) (Stefansson and Brautigan, 2006 ).
Genomic regions Chromosome 19
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PPP6R1-201 ENST00000412770.7 3984 881aa Protein coding CCDS46186 Q9UPN7 NM_014931.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PPP6R1-203 ENST00000587283.5 2953 881aa Protein coding CCDS46186 Q9UPN7 - TSL:1, GENCODE basic, APPRIS P1,
PPP6R1-208 ENST00000592242.1 574 157aa Protein coding - K7EM28 - CDS 3' incomplete, TSL:4,
PPP6R1-204 ENST00000587457.1 2389 No protein Retained intron - - - TSL:2,
PPP6R1-205 ENST00000589343.1 904 No protein Retained intron - - - TSL:2,
PPP6R1-206 ENST00000591323.5 848 No protein Retained intron - - - TSL:3,
PPP6R1-202 ENST00000586690.1 668 No protein Retained intron - - - TSL:2,
PPP6R1-207 ENST00000591602.1 520 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PPP6R1
This KO Strategy loading
Red Cotton™ Notes Gene PPP6R1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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