CRISPR-U™ Gene Knockout Cell Line
Strategy
PPP6R1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPP6R1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PPP6R1 |
| Gene id | 22870 |
| Organism | Homo sapiens |
| Official full symbol | protein phosphatase 6 regulatory subunit 1 |
| Gene type | protein-coding |
| Also known as | KIAA1115, PP6R1, SAP190, SAPS1 |
| Summary | Protein phosphatase regulatory subunits, such as SAPS1, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. SAPS1 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C; MIM 612725) (Stefansson and Brautigan, 2006 ). |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PPP6R1-201 | ENST00000412770.7 | 3984 | 881aa | Protein coding | CCDS46186 | Q9UPN7 | NM_014931.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PPP6R1-203 | ENST00000587283.5 | 2953 | 881aa | Protein coding | CCDS46186 | Q9UPN7 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PPP6R1-208 | ENST00000592242.1 | 574 | 157aa | Protein coding | - | K7EM28 | - | CDS 3' incomplete, TSL:4, |
| PPP6R1-204 | ENST00000587457.1 | 2389 | No protein | Retained intron | - | - | - | TSL:2, |
| PPP6R1-205 | ENST00000589343.1 | 904 | No protein | Retained intron | - | - | - | TSL:2, |
| PPP6R1-206 | ENST00000591323.5 | 848 | No protein | Retained intron | - | - | - | TSL:3, |
| PPP6R1-202 | ENST00000586690.1 | 668 | No protein | Retained intron | - | - | - | TSL:2, |
| PPP6R1-207 | ENST00000591602.1 | 520 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PPP6R1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PPP6R1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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