CRISPR-U™ Gene Knockout Cell Line Strategy

ATG14 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ATG14 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ATG14
Gene id 22863
Organism Homo sapiens
Official full symbol autophagy related 14
Gene type protein-coding
Also known as ATG14L, BARKOR, KIAA0831
Genomic regions Chromosome 14
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ATG14-201 ENST00000247178.6 4715 492aa Protein coding CCDS32087 Q6ZNE5-1 NM_014924.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ATG14-202 ENST00000558189.1 4689 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ATG14
This KO Strategy loading
Red Cotton™ Notes Gene ATG14 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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