CRISPR-U™ Gene Knockout Cell Line Strategy

LMTK2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LMTK2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LMTK2
Gene id 22853
Organism Homo sapiens
Official full symbol lemur tyrosine kinase 2
Gene type protein-coding
Also known as AATYK2, BREK, KPI-2, KPI2, LMR2, PPP1R100, cprk, hBREK
Summary The protein encoded by this gene belongs to the protein kinase superfamily and the protein tyrosine kinase family. It contains N-terminal transmembrane helices and a long C-terminal cytoplasmic tail with serine/threonine/tyrosine kinase activity. This protein interacts with several other proteins, such as Inhibitor-2 (Inh2), protein phosphatase-1 (PP1C), p35, and myosin VI. It phosporylates other proteins, and is itself also phosporylated when interacting with cyclin-dependent kinase 5 (cdk5)/p35 complex. This protein involves in nerve growth factor (NGF)-TrkA signalling, and also plays a critical role in endosomal membrane trafficking. Mouse studies suggested an essential role of this protein in spermatogenesis.
Genomic regions Chromosome 7
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LMTK2-201 ENST00000297293.6 8974 1503aa Protein coding CCDS5654 Q8IWU2 NM_014916.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LMTK2-202 ENST00000493372.1 540 No protein Processed transcript - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LMTK2
This KO Strategy loading
Red Cotton™ Notes Gene LMTK2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×

Comment: