CRISPR-U™ Gene Knockout Cell Line
Strategy
LMTK2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LMTK2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LMTK2 |
| Gene id | 22853 |
| Organism | Homo sapiens |
| Official full symbol | lemur tyrosine kinase 2 |
| Gene type | protein-coding |
| Also known as | AATYK2, BREK, KPI-2, KPI2, LMR2, PPP1R100, cprk, hBREK |
| Summary | The protein encoded by this gene belongs to the protein kinase superfamily and the protein tyrosine kinase family. It contains N-terminal transmembrane helices and a long C-terminal cytoplasmic tail with serine/threonine/tyrosine kinase activity. This protein interacts with several other proteins, such as Inhibitor-2 (Inh2), protein phosphatase-1 (PP1C), p35, and myosin VI. It phosporylates other proteins, and is itself also phosporylated when interacting with cyclin-dependent kinase 5 (cdk5)/p35 complex. This protein involves in nerve growth factor (NGF)-TrkA signalling, and also plays a critical role in endosomal membrane trafficking. Mouse studies suggested an essential role of this protein in spermatogenesis. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LMTK2-201 | ENST00000297293.6 | 8974 | 1503aa | Protein coding | CCDS5654 | Q8IWU2 | NM_014916.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LMTK2-202 | ENST00000493372.1 | 540 | No protein | Processed transcript | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LMTK2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LMTK2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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