CRISPR-U™ Gene Knockout Cell Line Strategy

MRAS Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MRAS Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MRAS
Gene id 22808
Organism Homo sapiens
Official full symbol muscle RAS oncogene homolog
Gene type protein-coding
Also known as M-RAs, NS11, R-RAS3, RRAS3
Summary This gene encodes a member of the Ras family of small GTPases. These membrane-associated proteins function as signal transducers in multiple processes including cell growth and differentiation, and dysregulation of Ras signaling has been associated with many types of cancer. The encoded protein may play a role in the tumor necrosis factor-alpha and MAP kinase signaling pathways. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Genomic regions Chromosome 3
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MRAS-201 ENST00000289104.8 4538 208aa Protein coding CCDS3100 O14807-1 - TSL:2, GENCODE basic, APPRIS P1,
MRAS-210 ENST00000614350.4 4377 132aa Protein coding CCDS58855 O14807-2 - TSL:2, GENCODE basic,
MRAS-202 ENST00000423968.7 4098 208aa Protein coding CCDS3100 O14807-1 NM_001085049.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MRAS-211 ENST00000621127.4 3801 132aa Protein coding CCDS58855 O14807-2 - TSL:5, GENCODE basic,
MRAS-204 ENST00000464896.5 1534 132aa Protein coding CCDS58855 O14807-2 - TSL:2, GENCODE basic,
MRAS-205 ENST00000474559.1 856 208aa Protein coding CCDS3100 O14807-1 - TSL:3, GENCODE basic, APPRIS P1,
MRAS-209 ENST00000494949.5 431 107aa Protein coding - C9J8Q6 - CDS 3' incomplete, TSL:5,
MRAS-206 ENST00000475711.5 271 23aa Protein coding - C9J601 - CDS 3' incomplete, TSL:3,
MRAS-203 ENST00000461114.1 973 No protein Processed transcript - - - TSL:2,
MRAS-207 ENST00000477784.1 394 No protein Processed transcript - - - TSL:3,
MRAS-208 ENST00000478647.1 429 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MRAS
This KO Strategy loading
Red Cotton™ Notes Gene MRAS had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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