CRISPR-U™ Gene Knockout Cell Line
Strategy
ITGA11 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ITGA11 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ITGA11 |
| Gene id | 22801 |
| Organism | Homo sapiens |
| Official full symbol | integrin subunit alpha 11 |
| Gene type | protein-coding |
| Also known as | HsT18964 |
| Summary | This gene encodes an alpha integrin. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein contains an I domain, is expressed in muscle tissue, dimerizes with beta 1 integrin in vitro, and appears to bind collagen in this form. Therefore, the protein may be involved in attaching muscle tissue to the extracellular matrix. Alternative transcriptional splice variants have been found for this gene, but their biological validity is not determined. |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ITGA11-201 | ENST00000315757.9 | 10191 | 1188aa | Protein coding | CCDS45291 | Q9UKX5-1 | NM_001004439.2 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| ITGA11-202 | ENST00000423218.6 | 10192 | 1189aa | Protein coding | - | Q9UKX5-2 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| ITGA11-203 | ENST00000562826.5 | 670 | No protein | Processed transcript | - | - | - | TSL:3, |
| ITGA11-205 | ENST00000566429.1 | 567 | No protein | Processed transcript | - | - | - | TSL:4, |
| ITGA11-207 | ENST00000569346.5 | 783 | No protein | Retained intron | - | - | - | TSL:4, |
| ITGA11-206 | ENST00000568677.1 | 651 | No protein | Retained intron | - | - | - | TSL:3, |
| ITGA11-204 | ENST00000565868.5 | 401 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ITGA11 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ITGA11 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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