CRISPR-U™ Gene Knockout Cell Line
Strategy
FHL2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FHL2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FHL2 |
| Gene id | 2274 |
| Organism | Homo sapiens |
| Official full symbol | four and a half LIM domains 2 |
| Gene type | protein-coding |
| Also known as | AAG11, DRAL, FHL-2, SLIM-3, SLIM3 |
| Summary | This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FHL2-207 | ENST00000409177.6 | 4881 | 279aa | Protein coding | CCDS2070 | Q14192-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| FHL2-202 | ENST00000344213.9 | 1876 | 279aa | Protein coding | CCDS2070 | Q14192-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| FHL2-208 | ENST00000409807.5 | 1601 | 279aa | Protein coding | CCDS2070 | Q14192-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| FHL2-211 | ENST00000530340.6 | 1582 | 279aa | Protein coding | CCDS2070 | Q14192-1 | NM_001318895.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| FHL2-205 | ENST00000393353.7 | 1531 | 279aa | Protein coding | CCDS2070 | Q14192-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| FHL2-201 | ENST00000322142.13 | 1478 | 279aa | Protein coding | CCDS2070 | Q14192-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| FHL2-204 | ENST00000393352.7 | 1426 | 279aa | Protein coding | CCDS2070 | Q14192-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| FHL2-206 | ENST00000408995.5 | 1367 | 279aa | Protein coding | CCDS2070 | Q14192-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| FHL2-203 | ENST00000358129.8 | 1578 | 151aa | Protein coding | - | Q14192-2 | - | TSL:5, GENCODE basic, |
| FHL2-212 | ENST00000607522.1 | 759 | 65aa | Protein coding | - | U3KQT4 | - | TSL:2, GENCODE basic, |
| FHL2-209 | ENST00000447958.1 | 574 | 19aa | Protein coding | - | C9J3S8 | - | CDS 3' incomplete, TSL:4, |
| FHL2-210 | ENST00000452732.1 | 886 | 54aa | Nonsense mediated decay | - | F8WDA8 | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FHL2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FHL2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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