CRISPR-U™ Gene Knockout Cell Line Strategy

FHL1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human FHL1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FHL1
Gene id 2273
Organism Homo sapiens
Official full symbol four and a half LIM domains 1
Gene type protein-coding
Summary This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.
Genomic regions Chromosome X
Strategy Summary
This gene has 31 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FHL1-216 ENST00000543669.5 2878 280aa Protein coding CCDS14655 Q13642-1 - TSL:1, GENCODE basic,
FHL1-232 ENST00000651089.1 2801 323aa Protein coding CCDS55507 Q13642-2 - GENCODE basic,
FHL1-205 ENST00000394155.8 2568 323aa Protein coding CCDS55507 Q13642-2 NM_001159702.3 TSL:5, GENCODE basic, MANE Plus Clinical v0.92,
FHL1-234 ENST00000651929.2 2441 280aa Protein coding CCDS14655 Q13642-1 - GENCODE basic,
FHL1-204 ENST00000394153.6 2374 280aa Protein coding CCDS14655 Q13642-1 - TSL:5, GENCODE basic,
FHL1-214 ENST00000535737.5 2325 280aa Protein coding CCDS14655 Q13642-1 - TSL:3, GENCODE basic,
FHL1-215 ENST00000539015.5 2310 309aa Protein coding CCDS55505 Q13642-4 - TSL:2, GENCODE basic,
FHL1-225 ENST00000628568.1 2304 280aa Protein coding CCDS14655 Q13642-1 - TSL:5, GENCODE basic,
FHL1-203 ENST00000370683.6 2286 296aa Protein coding CCDS55506 Q13642-5 NM_001159699.2 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
FHL1-217 ENST00000618438.4 2178 194aa Protein coding CCDS76036 Q13642-3 - TSL:3, GENCODE basic,
FHL1-227 ENST00000629039.2 1501 280aa Protein coding CCDS14655 Q13642-1 - TSL:2, GENCODE basic,
FHL1-228 ENST00000630084.2 1335 280aa Protein coding CCDS14655 Q13642-1 - TSL:2, GENCODE basic,
FHL1-226 ENST00000628919.3 1222 280aa Protein coding CCDS14655 Q13642-1 - TSL:5, GENCODE basic,
FHL1-202 ENST00000370676.7 833 210aa Protein coding CCDS83493 Q5JXI2 - TSL:5, GENCODE basic,
FHL1-235 ENST00000652457.1 2415 257aa Protein coding - A0A494C0D6 - GENCODE basic,
FHL1-201 ENST00000370674.3 1017 257aa Protein coding - Q5JXI8 - CDS 3' incomplete, TSL:5,
FHL1-206 ENST00000420362.5 926 204aa Protein coding - Q5JXH8 - CDS 3' incomplete, TSL:2,
FHL1-207 ENST00000434885.5 796 207aa Protein coding - Q5JXI3 - CDS 3' incomplete, TSL:2,
FHL1-209 ENST00000452016.5 796 207aa Protein coding - Q5JXI3 - CDS 3' incomplete, TSL:5,
FHL1-210 ENST00000456445.5 775 205aa Protein coding - Q5JXH7 - CDS 3' incomplete, TSL:2,
FHL1-211 ENST00000458357.5 629 155aa Protein coding - Q5JXH9 - CDS 3' incomplete, TSL:4,
FHL1-221 ENST00000627578.2 580 152aa Protein coding - A0A0D9SFB0 - CDS 3' incomplete, TSL:4,
FHL1-223 ENST00000628032.2 578 175aa Protein coding - A0A0D9SFZ9 - CDS 3' incomplete, TSL:5,
FHL1-224 ENST00000628443.2 578 147aa Protein coding - A0A0D9SFI6 - CDS 3' incomplete, TSL:4,
FHL1-229 ENST00000630278.2 567 78aa Protein coding - A0A0D9SGD1 - CDS 3' incomplete, TSL:5,
FHL1-220 ENST00000627383.2 566 138aa Protein coding - A0A0D9SGB2 - CDS 3' incomplete, TSL:4,
FHL1-208 ENST00000449474.5 539 141aa Protein coding - Q5JXI0 - CDS 3' incomplete, TSL:2,
FHL1-236 ENST00000652745.1 495 67aa Protein coding - A0A494C0J3 - CDS 3' incomplete,
FHL1-222 ENST00000627812.2 457 66aa Protein coding - A0A0D9SEY7 - CDS 3' incomplete, TSL:3,
FHL1-233 ENST00000651256.1 447 36aa Protein coding - A0A494C1G1 - CDS 3' incomplete,
FHL1-218 ENST00000625935.2 416 18aa Protein coding - A0A0D9SGC5 - CDS 3' incomplete, TSL:3,
FHL1-231 ENST00000630684.2 579 No protein Processed transcript - - - TSL:5,
FHL1-219 ENST00000626004.1 485 No protein Processed transcript - - - TSL:4,
FHL1-212 ENST00000477080.1 859 No protein Retained intron - - - TSL:2,
FHL1-213 ENST00000477204.5 790 No protein Retained intron - - - TSL:2,
FHL1-230 ENST00000630677.1 756 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FHL1
This KO Strategy loading
Red Cotton™ Notes Gene FHL1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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