CRISPR-U™ Gene Knockout Cell Line Strategy

FGFR1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human FGFR1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FGFR1
Gene id 2260
Organism Homo sapiens
Official full symbol fibroblast growth factor receptor 1
Gene type protein-coding
Summary The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
Genomic regions Chromosome 8
Strategy Summary
This gene has 20 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FGFR1-206 ENST00000397091.9 5702 820aa Protein coding CCDS43732 P11362-7 - TSL:1, GENCODE basic, APPRIS ALT1,
FGFR1-214 ENST00000447712.7 5697 822aa Protein coding CCDS6107 P11362-1 NM_023110.3 TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
FGFR1-238 ENST00000532791.5 5590 820aa Protein coding CCDS55222 P11362-2 - TSL:5, GENCODE basic, APPRIS P4,
FGFR1-211 ENST00000425967.7 5375 853aa Protein coding CCDS55223 P11362-21 - TSL:1, GENCODE basic,
FGFR1-202 ENST00000335922.9 4157 812aa Protein coding CCDS55221 P11362-20 - TSL:1, GENCODE basic,
FGFR1-204 ENST00000356207.9 3824 733aa Protein coding CCDS43730 P11362-3 - TSL:1, GENCODE basic,
FGFR1-201 ENST00000326324.10 3816 731aa Protein coding CCDS43731 P11362-14 - TSL:1, GENCODE basic,
FGFR1-209 ENST00000397113.6 3680 820aa Protein coding CCDS43732 P11362-7 - TSL:2, GENCODE basic, APPRIS ALT1,
FGFR1-208 ENST00000397108.8 2870 820aa Protein coding CCDS43732 P11362-7 - TSL:1, GENCODE basic, APPRIS ALT1,
FGFR1-203 ENST00000341462.8 6054 367aa Protein coding - C1KBH7 - TSL:5, GENCODE basic,
FGFR1-242 ENST00000619564.3 5482 228aa Protein coding - B5A958 - TSL:5, GENCODE basic,
FGFR1-207 ENST00000397103.5 2953 733aa Protein coding - E7EU09 - TSL:5, GENCODE basic,
FGFR1-226 ENST00000525001.5 1155 298aa Protein coding - E9PNM3 - CDS 3' incomplete, TSL:5,
FGFR1-233 ENST00000529552.5 688 142aa Protein coding - E9PKV7 - CDS 3' incomplete, TSL:4,
FGFR1-229 ENST00000526742.5 657 145aa Protein coding - E9PKF2 - CDS 3' incomplete, TSL:4,
FGFR1-210 ENST00000413133.6 587 106aa Protein coding - C9J205 - CDS 3' incomplete, TSL:4,
FGFR1-241 ENST00000533668.5 571 139aa Protein coding - E9PN14 - CDS 3' incomplete, TSL:5,
FGFR1-212 ENST00000434187.5 529 54aa Protein coding - C9J1L5 - CDS 3' incomplete, TSL:4,
FGFR1-234 ENST00000530568.5 467 85aa Protein coding - E9PQ40 - CDS 3' incomplete, TSL:3,
FGFR1-213 ENST00000440174.1 389 1aa Protein coding - - - CDS 3' incomplete, TSL:3,
FGFR1-243 ENST00000649678.1 6216 818aa Nonsense mediated decay - A0A3B3ISD1 - -
FGFR1-247 ENST00000674380.1 5002 42aa Nonsense mediated decay - A0A6I8PTV4 - -
FGFR1-244 ENST00000674189.1 3309 42aa Nonsense mediated decay - A0A6I8PTV4 - -
FGFR1-222 ENST00000487647.5 1608 61aa Nonsense mediated decay - E9PKX3 - TSL:1,
FGFR1-246 ENST00000674235.1 1071 245aa Nonsense mediated decay - A0A6I8PRY1 - CDS 5' incomplete,
FGFR1-236 ENST00000531196.5 842 134aa Nonsense mediated decay - H0YE20 - CDS 5' incomplete, TSL:3,
FGFR1-221 ENST00000484370.5 826 150aa Nonsense mediated decay - P11362-15 - TSL:1,
FGFR1-224 ENST00000496629.1 584 No protein Processed transcript - - - TSL:4,
FGFR1-228 ENST00000526688.1 570 No protein Processed transcript - - - TSL:3,
FGFR1-231 ENST00000527203.5 556 No protein Processed transcript - - - TSL:3,
FGFR1-220 ENST00000480571.1 442 No protein Processed transcript - - - TSL:4,
FGFR1-239 ENST00000533301.1 377 No protein Processed transcript - - - TSL:3,
FGFR1-235 ENST00000530701.1 276 No protein Processed transcript - - - TSL:1,
FGFR1-227 ENST00000526570.5 5528 No protein Retained intron - - - TSL:1,
FGFR1-248 ENST00000674474.1 4711 No protein Retained intron - - - -
FGFR1-217 ENST00000470826.5 2281 No protein Retained intron - - - TSL:1,
FGFR1-245 ENST00000674217.1 2011 No protein Retained intron - - - -
FGFR1-223 ENST00000496296.5 1898 No protein Retained intron - - - TSL:1,
FGFR1-230 ENST00000527114.5 1499 No protein Retained intron - - - TSL:5,
FGFR1-216 ENST00000466021.5 823 No protein Retained intron - - - TSL:2,
FGFR1-232 ENST00000527745.3 790 No protein Retained intron - - - TSL:3,
FGFR1-205 ENST00000397090.4 777 No protein Retained intron - - - TSL:2,
FGFR1-218 ENST00000474970.1 764 No protein Retained intron - - - TSL:5,
FGFR1-237 ENST00000532386.5 725 No protein Retained intron - - - TSL:3,
FGFR1-215 ENST00000464163.1 545 No protein Retained intron - - - TSL:4,
FGFR1-240 ENST00000533619.5 540 No protein Retained intron - - - TSL:4,
FGFR1-225 ENST00000524528.1 486 No protein Retained intron - - - TSL:3,
FGFR1-219 ENST00000475621.1 485 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FGFR1
This KO Strategy loading
Red Cotton™ Notes Gene FGFR1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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