CRISPR-U™ Gene Knockout Cell Line
Strategy
FGF11 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FGF11 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FGF11 |
| Gene id | 2256 |
| Organism | Homo sapiens |
| Official full symbol | fibroblast growth factor 11 |
| Gene type | protein-coding |
| Also known as | FGF-11, FHF-3, FHF3 |
| Summary | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this gene has not yet been determined. The expression pattern of the mouse homolog implies a role in nervous system development. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FGF11-201 | ENST00000293829.9 | 2578 | 225aa | Protein coding | CCDS11105 | Q92914 | NM_004112.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| FGF11-202 | ENST00000572907.5 | 2358 | 101aa | Protein coding | - | I3L4N4 | - | TSL:1, GENCODE basic, |
| FGF11-203 | ENST00000575082.5 | 2315 | 101aa | Protein coding | - | I3L4N4 | - | TSL:2, GENCODE basic, |
| FGF11-205 | ENST00000575398.5 | 990 | 101aa | Protein coding | - | I3L4N4 | - | TSL:2, GENCODE basic, |
| FGF11-204 | ENST00000575235.5 | 928 | 101aa | Protein coding | - | I3L4N4 | - | TSL:1, GENCODE basic, |
| FGF11-206 | ENST00000576328.1 | 912 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FGF11 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FGF11 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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