CRISPR-U™ Gene Knockout Cell Line
Strategy
LRWD1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LRWD1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LRWD1 |
| Gene id | 222229 |
| Organism | Homo sapiens |
| Official full symbol | leucine rich repeats and WD repeat domain containing 1 |
| Gene type | protein-coding |
| Also known as | CENP-33, ORCA |
| Summary | The protein encoded by this gene interacts with components of the origin recognition complex (ORC) and regulates the formation of the prereplicative complex. The encoded protein stabilizes the ORC and therefore aids in DNA replication. This protein is required for the G1/S phase transition of the cell cycle. In addition, the encoded protein binds to trimethylated histone H3 in heterochromatin and recruits the ORC and lysine methyltransferases, which help maintain the repressive heterochromatic state. Two transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LRWD1-201 | ENST00000292616.10 | 2160 | 647aa | Protein coding | CCDS34715 | A0A140VJD0 Q9UFC0 | NM_152892.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LRWD1-202 | ENST00000463739.5 | 1062 | 154aa | Protein coding | - | F8WDB4 | - | CDS 3' incomplete, TSL:5, |
| LRWD1-204 | ENST00000468175.1 | 847 | 242aa | Protein coding | - | H7C5N7 | - | CDS 5' incomplete, TSL:3, |
| LRWD1-209 | ENST00000626402.1 | 138 | 45aa | Protein coding | - | F8WBP5 | - | TSL:3, GENCODE basic, |
| LRWD1-208 | ENST00000485808.5 | 908 | 179aa | Nonsense mediated decay | - | H7C5S6 | - | CDS 5' incomplete, TSL:5, |
| LRWD1-206 | ENST00000476270.1 | 703 | 41aa | Nonsense mediated decay | - | H7C4Q9 | - | CDS 5' incomplete, TSL:5, |
| LRWD1-203 | ENST00000464107.5 | 646 | 45aa | Nonsense mediated decay | - | F8WBP5 | - | TSL:3, |
| LRWD1-205 | ENST00000473880.5 | 5491 | No protein | Retained intron | - | - | - | TSL:2, |
| LRWD1-207 | ENST00000485417.1 | 552 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LRWD1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LRWD1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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