CRISPR-U™ Gene Knockout Cell Line Strategy

LRWD1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LRWD1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LRWD1
Gene id 222229
Organism Homo sapiens
Official full symbol leucine rich repeats and WD repeat domain containing 1
Gene type protein-coding
Also known as CENP-33, ORCA
Summary The protein encoded by this gene interacts with components of the origin recognition complex (ORC) and regulates the formation of the prereplicative complex. The encoded protein stabilizes the ORC and therefore aids in DNA replication. This protein is required for the G1/S phase transition of the cell cycle. In addition, the encoded protein binds to trimethylated histone H3 in heterochromatin and recruits the ORC and lysine methyltransferases, which help maintain the repressive heterochromatic state. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 7
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LRWD1-201 ENST00000292616.10 2160 647aa Protein coding CCDS34715 A0A140VJD0 Q9UFC0 NM_152892.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LRWD1-202 ENST00000463739.5 1062 154aa Protein coding - F8WDB4 - CDS 3' incomplete, TSL:5,
LRWD1-204 ENST00000468175.1 847 242aa Protein coding - H7C5N7 - CDS 5' incomplete, TSL:3,
LRWD1-209 ENST00000626402.1 138 45aa Protein coding - F8WBP5 - TSL:3, GENCODE basic,
LRWD1-208 ENST00000485808.5 908 179aa Nonsense mediated decay - H7C5S6 - CDS 5' incomplete, TSL:5,
LRWD1-206 ENST00000476270.1 703 41aa Nonsense mediated decay - H7C4Q9 - CDS 5' incomplete, TSL:5,
LRWD1-203 ENST00000464107.5 646 45aa Nonsense mediated decay - F8WBP5 - TSL:3,
LRWD1-205 ENST00000473880.5 5491 No protein Retained intron - - - TSL:2,
LRWD1-207 ENST00000485417.1 552 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LRWD1
This KO Strategy loading
Red Cotton™ Notes Gene LRWD1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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