CRISPR-U™ Gene Knockout Cell Line Strategy

KDM1B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human KDM1B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol KDM1B
Gene id 221656
Organism Homo sapiens
Official full symbol lysine demethylase 1B
Gene type protein-coding
Also known as AOF1, C6orf193, LSD2
Summary Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 ).
Genomic regions Chromosome 6
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
KDM1B-201 ENST00000297792.9 3811 590aa Protein coding CCDS34343 Q8NB78-2 - TSL:2, GENCODE basic,
KDM1B-205 ENST00000650836.2 4538 822aa Protein coding - Q8NB78-1 NM_001364614.2 GENCODE basic, APPRIS P1, MANE Select v0.92,
KDM1B-202 ENST00000449850.2 2877 823aa Protein coding - H0Y6H0 - TSL:5, GENCODE basic,
KDM1B-203 ENST00000546309.6 496 113aa Protein coding - Q08EI0 - TSL:1, GENCODE basic,
KDM1B-204 ENST00000642162.1 3447 No protein Retained intron - - - -
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene KDM1B
This KO Strategy loading
Red Cotton™ Notes Gene KDM1B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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