CRISPR-U™ Gene Knockout Cell Line
Strategy
KDM1B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human KDM1B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | KDM1B |
| Gene id | 221656 |
| Organism | Homo sapiens |
| Official full symbol | lysine demethylase 1B |
| Gene type | protein-coding |
| Also known as | AOF1, C6orf193, LSD2 |
| Summary | Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 ). |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| KDM1B-201 | ENST00000297792.9 | 3811 | 590aa | Protein coding | CCDS34343 | Q8NB78-2 | - | TSL:2, GENCODE basic, |
| KDM1B-205 | ENST00000650836.2 | 4538 | 822aa | Protein coding | - | Q8NB78-1 | NM_001364614.2 | GENCODE basic, APPRIS P1, MANE Select v0.92, |
| KDM1B-202 | ENST00000449850.2 | 2877 | 823aa | Protein coding | - | H0Y6H0 | - | TSL:5, GENCODE basic, |
| KDM1B-203 | ENST00000546309.6 | 496 | 113aa | Protein coding | - | Q08EI0 | - | TSL:1, GENCODE basic, |
| KDM1B-204 | ENST00000642162.1 | 3447 | No protein | Retained intron | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | KDM1B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene KDM1B had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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