CRISPR-U™ Gene Knockout Cell Line
Strategy
HNRNPA3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HNRNPA3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HNRNPA3 |
| Gene id | 220988 |
| Organism | Homo sapiens |
| Official full symbol | heterogeneous nuclear ribonucleoprotein A3 |
| Gene type | protein-coding |
| Also known as | 2610510D13Rik, D10S102, FBRNP, HNRPA3 |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HNRNPA3-202 | ENST00000411529.6 | 5688 | 356aa | Protein coding | CCDS82536 | P51991-2 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| HNRNPA3-216 | ENST00000678111.1 | 3111 | 378aa | Protein coding | CCDS2273 | A0A384NL63 | - | GENCODE basic, APPRIS P2, |
| HNRNPA3-211 | ENST00000676874.1 | 3028 | 356aa | Protein coding | CCDS82536 | B4DDB6 | - | GENCODE basic, APPRIS ALT2, |
| HNRNPA3-201 | ENST00000392524.6 | 2067 | 378aa | Protein coding | CCDS2273 | P51991-1 | - | TSL:5, GENCODE basic, APPRIS P2, |
| HNRNPA3-204 | ENST00000435711.5 | 1731 | 378aa | Protein coding | CCDS2273 | P51991-1 | - | TSL:1, GENCODE basic, APPRIS P2, |
| HNRNPA3-215 | ENST00000677863.1 | 5651 | 348aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| HNRNPA3-209 | ENST00000676736.1 | 5626 | 348aa | Protein coding | - | - | - | GENCODE basic, |
| HNRNPA3-208 | ENST00000676681.1 | 5600 | 331aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| HNRNPA3-212 | ENST00000677043.1 | 2926 | 331aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| HNRNPA3-219 | ENST00000679328.1 | 1911 | 396aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| HNRNPA3-203 | ENST00000432457.2 | 643 | 115aa | Protein coding | - | H7C1J8 | - | CDS 5' incomplete, TSL:3, |
| HNRNPA3-207 | ENST00000676629.1 | 5750 | 378aa | Nonsense mediated decay | CCDS2273 | A0A384NL63 | - | - |
| HNRNPA3-218 | ENST00000679159.1 | 5697 | 378aa | Nonsense mediated decay | CCDS2273 | A0A384NL63 | - | - |
| HNRNPA3-213 | ENST00000677337.1 | 5654 | 377aa | Nonsense mediated decay | - | - | - | - |
| HNRNPA3-206 | ENST00000676488.1 | 5516 | 331aa | Nonsense mediated decay | - | - | - | - |
| HNRNPA3-210 | ENST00000676762.1 | 6209 | No protein | Retained intron | - | - | - | - |
| HNRNPA3-205 | ENST00000483137.2 | 6122 | No protein | Retained intron | - | - | - | TSL:2, |
| HNRNPA3-214 | ENST00000677508.1 | 5953 | No protein | Retained intron | - | - | - | - |
| HNRNPA3-217 | ENST00000678421.1 | 5829 | No protein | Retained intron | - | - | - | - |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HNRNPA3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HNRNPA3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: