CRISPR-U™ Gene Knockout Cell Line
Strategy
FCER2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FCER2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FCER2 |
| Gene id | 2208 |
| Organism | Homo sapiens |
| Official full symbol | Fc fragment of IgE receptor II |
| Gene type | protein-coding |
| Also known as | BLAST-2, CD23, CD23A, CLEC4J, FCE2, IGEBF |
| Summary | The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FCER2-201 | ENST00000346664.9 | 1596 | 321aa | Protein coding | CCDS12184 | P06734 | - | TSL:1, GENCODE basic, APPRIS P2, |
| FCER2-205 | ENST00000597921.6 | 1585 | 321aa | Protein coding | CCDS12184 | P06734 | NM_001220500.2 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| FCER2-202 | ENST00000360067.8 | 1482 | 320aa | Protein coding | - | K3W4U1 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| FCER2-203 | ENST00000593418.1 | 801 | 159aa | Protein coding | - | M0R1R5 | - | CDS 3' incomplete, TSL:3, |
| FCER2-204 | ENST00000597312.5 | 1910 | No protein | Retained intron | - | - | - | TSL:2, |
| FCER2-207 | ENST00000598803.5 | 1872 | No protein | Retained intron | - | - | - | TSL:2, |
| FCER2-206 | ENST00000597934.1 | 1577 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FCER2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FCER2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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