CRISPR-U™ Gene Knockout Cell Line Strategy

FCER2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human FCER2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FCER2
Gene id 2208
Organism Homo sapiens
Official full symbol Fc fragment of IgE receptor II
Gene type protein-coding
Also known as BLAST-2, CD23, CD23A, CLEC4J, FCE2, IGEBF
Summary The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Genomic regions Chromosome 19
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FCER2-201 ENST00000346664.9 1596 321aa Protein coding CCDS12184 P06734 - TSL:1, GENCODE basic, APPRIS P2,
FCER2-205 ENST00000597921.6 1585 321aa Protein coding CCDS12184 P06734 NM_001220500.2 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
FCER2-202 ENST00000360067.8 1482 320aa Protein coding - K3W4U1 - TSL:5, GENCODE basic, APPRIS ALT2,
FCER2-203 ENST00000593418.1 801 159aa Protein coding - M0R1R5 - CDS 3' incomplete, TSL:3,
FCER2-204 ENST00000597312.5 1910 No protein Retained intron - - - TSL:2,
FCER2-207 ENST00000598803.5 1872 No protein Retained intron - - - TSL:2,
FCER2-206 ENST00000597934.1 1577 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FCER2
This KO Strategy loading
Red Cotton™ Notes Gene FCER2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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