CRISPR-U™ Gene Knockout Cell Line Strategy

PATL1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PATL1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PATL1
Gene id 219988
Organism Homo sapiens
Official full symbol PAT1 homolog 1, processing body mRNA decay factor
Gene type protein-coding
Also known as Pat1b, hPat1b
Genomic regions Chromosome 11
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PATL1-201 ENST00000300146.10 4129 770aa Protein coding CCDS44613 Q86TB9-1 NM_152716.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PATL1-202 ENST00000531919.1 440 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PATL1
This KO Strategy loading
Red Cotton™ Notes Gene PATL1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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