CRISPR-U™ Gene Knockout Cell Line Strategy

ACSL3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ACSL3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ACSL3
Gene id 2181
Organism Homo sapiens
Official full symbol acyl-CoA synthetase long chain family member 3
Gene type protein-coding
Also known as ACS3, FACL3, LACS 3, LACS3, PRO2194
Summary The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.
Genomic regions Chromosome 2
Strategy Summary
This gene has 16 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ACSL3-201 ENST00000357430.8 5577 720aa Protein coding CCDS2455 A0A024R487 O95573 NM_004457.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ACSL3-212 ENST00000679514.1 5574 720aa Protein coding CCDS2455 A0A024R487 - GENCODE basic, APPRIS P1,
ACSL3-229 ENST00000680921.1 5544 720aa Protein coding CCDS2455 A0A024R487 - GENCODE basic, APPRIS P1,
ACSL3-222 ENST00000680395.1 5539 720aa Protein coding CCDS2455 A0A024R487 - GENCODE basic, APPRIS P1,
ACSL3-234 ENST00000681383.1 5537 720aa Protein coding CCDS2455 A0A024R487 - GENCODE basic, APPRIS P1,
ACSL3-235 ENST00000681697.1 5524 720aa Protein coding CCDS2455 A0A024R487 - GENCODE basic, APPRIS P1,
ACSL3-219 ENST00000680147.1 5500 720aa Protein coding CCDS2455 A0A024R487 - GENCODE basic, APPRIS P1,
ACSL3-216 ENST00000679558.1 5464 720aa Protein coding CCDS2455 A0A024R487 - GENCODE basic, APPRIS P1,
ACSL3-220 ENST00000680251.1 5433 720aa Protein coding CCDS2455 A0A024R487 - GENCODE basic, APPRIS P1,
ACSL3-227 ENST00000680684.1 5341 720aa Protein coding CCDS2455 A0A024R487 - GENCODE basic, APPRIS P1,
ACSL3-202 ENST00000392066.7 3147 720aa Protein coding CCDS2455 A0A024R487 O95573 - TSL:5, GENCODE basic, APPRIS P1,
ACSL3-214 ENST00000679545.1 5468 712aa Protein coding - - - GENCODE basic,
ACSL3-213 ENST00000679541.1 5417 695aa Protein coding - - - GENCODE basic,
ACSL3-230 ENST00000681009.1 5022 568aa Protein coding - - - GENCODE basic,
ACSL3-204 ENST00000413316.1 650 88aa Protein coding - C9JC11 - CDS 3' incomplete, TSL:4,
ACSL3-210 ENST00000540115.1 624 141aa Protein coding - F5GWH2 - CDS 3' incomplete, TSL:3,
ACSL3-236 ENST00000681906.1 6794 518aa Nonsense mediated decay - - - -
ACSL3-223 ENST00000680420.1 5680 659aa Nonsense mediated decay - - - -
ACSL3-225 ENST00000680475.1 5652 518aa Nonsense mediated decay - - - -
ACSL3-226 ENST00000680525.1 5545 518aa Nonsense mediated decay - - - -
ACSL3-228 ENST00000680736.1 5479 283aa Nonsense mediated decay - - - -
ACSL3-232 ENST00000681292.1 5341 283aa Nonsense mediated decay - - - -
ACSL3-203 ENST00000407441.5 5244 434aa Nonsense mediated decay - H7BYZ7 - TSL:3,
ACSL3-224 ENST00000680424.1 5311 No protein Processed transcript - - - -
ACSL3-215 ENST00000679546.1 2131 No protein Processed transcript - - - -
ACSL3-205 ENST00000421680.2 639 No protein Processed transcript - - - TSL:3,
ACSL3-217 ENST00000679932.1 8441 No protein Retained intron - - - -
ACSL3-218 ENST00000680100.1 6769 No protein Retained intron - - - -
ACSL3-233 ENST00000681326.1 6741 No protein Retained intron - - - -
ACSL3-231 ENST00000681017.1 4538 No protein Retained intron - - - -
ACSL3-221 ENST00000680382.1 1900 No protein Retained intron - - - -
ACSL3-209 ENST00000535678.2 1762 No protein Retained intron - - - TSL:3,
ACSL3-207 ENST00000474422.1 760 No protein Retained intron - - - TSL:3,
ACSL3-208 ENST00000495541.1 749 No protein Retained intron - - - TSL:2,
ACSL3-206 ENST00000463813.1 509 No protein Retained intron - - - TSL:3,
ACSL3-211 ENST00000542810.1 428 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ACSL3
This KO Strategy loading
Red Cotton™ Notes Gene ACSL3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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