CRISPR-U™ Gene Knockout Cell Line
Strategy
ACSL1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ACSL1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ACSL1 |
| Gene id | 2180 |
| Organism | Homo sapiens |
| Official full symbol | acyl-CoA synthetase long chain family member 1 |
| Gene type | protein-coding |
| Also known as | ACS1, FACL1, FACL2, LACS, LACS1, LACS2 |
| Summary | The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ACSL1-211 | ENST00000515030.5 | 3948 | 698aa | Protein coding | CCDS3839 | P33121-1 | - | TSL:5, GENCODE basic, APPRIS P3, |
| ACSL1-201 | ENST00000281455.7 | 3712 | 698aa | Protein coding | CCDS3839 | P33121-1 | NM_001995.5 | TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92, |
| ACSL1-202 | ENST00000454703.6 | 3636 | 527aa | Protein coding | CCDS75213 | B7Z3Z9 | - | TSL:5, GENCODE basic, |
| ACSL1-208 | ENST00000507295.5 | 2490 | 664aa | Protein coding | CCDS68825 | E7EPM6 | - | TSL:2, GENCODE basic, |
| ACSL1-210 | ENST00000513317.5 | 2469 | 698aa | Protein coding | CCDS68826 | P33121-3 | - | TSL:2, GENCODE basic, APPRIS ALT1, |
| ACSL1-204 | ENST00000504342.5 | 2333 | 698aa | Protein coding | CCDS3839 | P33121-1 | - | TSL:1, GENCODE basic, APPRIS P3, |
| ACSL1-203 | ENST00000503407.5 | 2409 | 294aa | Protein coding | - | H0Y9U7 | - | CDS 5' incomplete, TSL:2, |
| ACSL1-205 | ENST00000504900.5 | 1583 | 388aa | Protein coding | - | D6RER0 | - | TSL:5, GENCODE basic, |
| ACSL1-206 | ENST00000505492.1 | 280 | 94aa | Protein coding | - | H0Y9Z9 | - | CDS 5' and 3' incomplete, TSL:1, |
| ACSL1-207 | ENST00000506733.5 | 2646 | 126aa | Nonsense mediated decay | - | D6RG07 | - | TSL:2, |
| ACSL1-209 | ENST00000513001.5 | 2796 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ACSL1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ACSL1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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