CRISPR-U™ Gene Knockout Cell Line Strategy

ACSL1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ACSL1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ACSL1
Gene id 2180
Organism Homo sapiens
Official full symbol acyl-CoA synthetase long chain family member 1
Gene type protein-coding
Also known as ACS1, FACL1, FACL2, LACS, LACS1, LACS2
Summary The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 4
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ACSL1-211 ENST00000515030.5 3948 698aa Protein coding CCDS3839 P33121-1 - TSL:5, GENCODE basic, APPRIS P3,
ACSL1-201 ENST00000281455.7 3712 698aa Protein coding CCDS3839 P33121-1 NM_001995.5 TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92,
ACSL1-202 ENST00000454703.6 3636 527aa Protein coding CCDS75213 B7Z3Z9 - TSL:5, GENCODE basic,
ACSL1-208 ENST00000507295.5 2490 664aa Protein coding CCDS68825 E7EPM6 - TSL:2, GENCODE basic,
ACSL1-210 ENST00000513317.5 2469 698aa Protein coding CCDS68826 P33121-3 - TSL:2, GENCODE basic, APPRIS ALT1,
ACSL1-204 ENST00000504342.5 2333 698aa Protein coding CCDS3839 P33121-1 - TSL:1, GENCODE basic, APPRIS P3,
ACSL1-203 ENST00000503407.5 2409 294aa Protein coding - H0Y9U7 - CDS 5' incomplete, TSL:2,
ACSL1-205 ENST00000504900.5 1583 388aa Protein coding - D6RER0 - TSL:5, GENCODE basic,
ACSL1-206 ENST00000505492.1 280 94aa Protein coding - H0Y9Z9 - CDS 5' and 3' incomplete, TSL:1,
ACSL1-207 ENST00000506733.5 2646 126aa Nonsense mediated decay - D6RG07 - TSL:2,
ACSL1-209 ENST00000513001.5 2796 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ACSL1
This KO Strategy loading
Red Cotton™ Notes Gene ACSL1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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