CRISPR-U™ Gene Knockout Cell Line Strategy

FANCD2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human FANCD2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FANCD2
Gene id 2177
Organism Homo sapiens
Official full symbol FA complementation group D2
Gene type protein-coding
Also known as FA-D2, FA4, FACD, FAD, FAD2, FANCD
Summary The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 3
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FANCD2-201 ENST00000287647.7 5219 1471aa Protein coding CCDS2595 Q9BXW9-1 - TSL:1, GENCODE basic, APPRIS ALT2,
FANCD2-202 ENST00000419585.5 5185 1451aa Protein coding CCDS33696 Q9BXW9-2 - TSL:1, GENCODE basic, APPRIS P4,
FANCD2-213 ENST00000675286.1 5096 1451aa Protein coding CCDS33696 - NM_001018115.3 GENCODE basic, APPRIS ALT2, MANE Select v0.92,
FANCD2-214 ENST00000676013.1 4334 1414aa Protein coding - - - CDS 3' incomplete,
FANCD2-204 ENST00000431693.1 2450 241aa Protein coding - Q9BXW9-4 - TSL:1, GENCODE basic,
FANCD2-212 ENST00000625535.1 117 38aa Protein coding - F8WE37 - TSL:5, GENCODE basic,
FANCD2-203 ENST00000421731.5 3425 749aa Nonsense mediated decay - H7BZJ7 - CDS 5' incomplete, TSL:1,
FANCD2-205 ENST00000435522.5 567 38aa Nonsense mediated decay - F8WE37 - TSL:4,
FANCD2-206 ENST00000438741.1 558 No protein Processed transcript - - - TSL:2,
FANCD2-209 ENST00000470757.5 547 No protein Processed transcript - - - TSL:2,
FANCD2-211 ENST00000483276.1 505 No protein Processed transcript - - - TSL:4,
FANCD2-208 ENST00000470028.1 449 No protein Processed transcript - - - TSL:5,
FANCD2-210 ENST00000480909.1 603 No protein Retained intron - - - TSL:2,
FANCD2-207 ENST00000464934.1 563 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FANCD2
This KO Strategy loading
Red Cotton™ Notes Gene FANCD2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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