CRISPR-U™ Gene Knockout Cell Line Strategy

F2R Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human F2R Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol F2R
Gene id 2149
Organism Homo sapiens
Official full symbol coagulation factor II thrombin receptor
Gene type protein-coding
Also known as CF2R, HTR, PAR-1, PAR1, TR
Summary Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 5
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
F2R-201 ENST00000319211.5 3727 425aa Protein coding CCDS4032 A0A024RAP7 P25116 NM_001992.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
F2R-202 ENST00000505600.1 442 102aa Protein coding - G3XAL6 - TSL:2, GENCODE basic,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene F2R
This KO Strategy loading
Red Cotton™ Notes Gene F2R had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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