CRISPR-U™ Gene Knockout Cell Line
Strategy
F2R Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human F2R Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | F2R |
| Gene id | 2149 |
| Organism | Homo sapiens |
| Official full symbol | coagulation factor II thrombin receptor |
| Gene type | protein-coding |
| Also known as | CF2R, HTR, PAR-1, PAR1, TR |
| Summary | Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| F2R-201 | ENST00000319211.5 | 3727 | 425aa | Protein coding | CCDS4032 | A0A024RAP7 P25116 | NM_001992.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| F2R-202 | ENST00000505600.1 | 442 | 102aa | Protein coding | - | G3XAL6 | - | TSL:2, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | F2R |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene F2R had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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