CRISPR-U™ Gene Knockout Cell Line
Strategy
ETV5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ETV5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ETV5 |
| Gene id | 2119 |
| Organism | Homo sapiens |
| Official full symbol | ETS variant transcription factor 5 |
| Gene type | protein-coding |
| Also known as | ERM |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ETV5-201 | ENST00000306376.10 | 4082 | 510aa | Protein coding | CCDS33906 | P41161-1 | NM_004454.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ETV5-206 | ENST00000434744.5 | 2213 | 510aa | Protein coding | CCDS33906 | P41161-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| ETV5-207 | ENST00000440773.5 | 574 | 150aa | Protein coding | - | C9JMF0 | - | CDS 3' incomplete, TSL:4, |
| ETV5-202 | ENST00000413301.5 | 573 | 132aa | Protein coding | - | C9J5A5 | - | CDS 3' incomplete, TSL:4, |
| ETV5-204 | ENST00000422039.1 | 564 | 132aa | Protein coding | - | C9JR66 | - | CDS 3' incomplete, TSL:4, |
| ETV5-203 | ENST00000421809.5 | 554 | 140aa | Protein coding | - | C9J0Z2 | - | CDS 3' incomplete, TSL:2, |
| ETV5-205 | ENST00000433149.1 | 366 | 89aa | Nonsense mediated decay | - | H7C1D2 | - | CDS 5' incomplete, TSL:5, |
| ETV5-211 | ENST00000480706.1 | 764 | No protein | Processed transcript | - | - | - | TSL:3, |
| ETV5-210 | ENST00000476890.1 | 642 | No protein | Processed transcript | - | - | - | TSL:2, |
| ETV5-213 | ENST00000489830.5 | 614 | No protein | Processed transcript | - | - | - | TSL:2, |
| ETV5-212 | ENST00000484223.1 | 590 | No protein | Processed transcript | - | - | - | TSL:4, |
| ETV5-208 | ENST00000472868.6 | 238 | No protein | Processed transcript | - | - | - | TSL:5, |
| ETV5-209 | ENST00000475484.1 | 472 | No protein | Retained intron | - | - | - | TSL:3, |
| ETV5-214 | ENST00000495808.1 | 187 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ETV5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ETV5 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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