CRISPR-U™ Gene Knockout Cell Line
Strategy
ERN1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ERN1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ERN1 |
| Gene id | 2081 |
| Organism | Homo sapiens |
| Official full symbol | endoplasmic reticulum to nucleus signaling 1 |
| Gene type | protein-coding |
| Also known as | IRE1, IRE1P, IRE1a, hIRE1p |
| Summary | This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ERN1-201 | ENST00000433197.4 | 7895 | 977aa | Protein coding | CCDS45762 | O75460-1 | NM_001433.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ERN1-208 | ENST00000680433.1 | 9028 | 905aa | Protein coding | - | - | - | GENCODE basic, |
| ERN1-209 | ENST00000680493.1 | 4833 | 96aa | Protein coding | - | - | - | GENCODE basic, |
| ERN1-207 | ENST00000606895.2 | 4717 | 70aa | Protein coding | - | O75460-2 | - | TSL:NA, GENCODE basic, |
| ERN1-210 | ENST00000680625.1 | 7687 | No protein | Processed transcript | - | - | - | - |
| ERN1-202 | ENST00000577567.5 | 398 | No protein | Processed transcript | - | - | - | TSL:5, |
| ERN1-205 | ENST00000583896.1 | 571 | No protein | Retained intron | - | - | - | TSL:5, |
| ERN1-206 | ENST00000584041.1 | 566 | No protein | Retained intron | - | - | - | TSL:4, |
| ERN1-203 | ENST00000579249.1 | 548 | No protein | Retained intron | - | - | - | TSL:3, |
| ERN1-204 | ENST00000583077.1 | 503 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ERN1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ERN1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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