CRISPR-U™ Gene Knockout Cell Line Strategy

ERN1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ERN1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ERN1
Gene id 2081
Organism Homo sapiens
Official full symbol endoplasmic reticulum to nucleus signaling 1
Gene type protein-coding
Also known as IRE1, IRE1P, IRE1a, hIRE1p
Summary This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
Genomic regions Chromosome 17
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ERN1-201 ENST00000433197.4 7895 977aa Protein coding CCDS45762 O75460-1 NM_001433.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ERN1-208 ENST00000680433.1 9028 905aa Protein coding - - - GENCODE basic,
ERN1-209 ENST00000680493.1 4833 96aa Protein coding - - - GENCODE basic,
ERN1-207 ENST00000606895.2 4717 70aa Protein coding - O75460-2 - TSL:NA, GENCODE basic,
ERN1-210 ENST00000680625.1 7687 No protein Processed transcript - - - -
ERN1-202 ENST00000577567.5 398 No protein Processed transcript - - - TSL:5,
ERN1-205 ENST00000583896.1 571 No protein Retained intron - - - TSL:5,
ERN1-206 ENST00000584041.1 566 No protein Retained intron - - - TSL:4,
ERN1-203 ENST00000579249.1 548 No protein Retained intron - - - TSL:3,
ERN1-204 ENST00000583077.1 503 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ERN1
This KO Strategy loading
Red Cotton™ Notes Gene ERN1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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