CRISPR-U™ Gene Knockout Cell Line Strategy

EPHB4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human EPHB4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol EPHB4
Gene id 2050
Organism Homo sapiens
Official full symbol EPH receptor B4
Gene type protein-coding
Also known as CMAVM2, HFASD, HTK, LMPHM7, MYK1, TYRO11
Summary Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development.
Genomic regions Chromosome 7
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
EPHB4-201 ENST00000358173.8 4353 987aa Protein coding CCDS5706 P54760-1 NM_004444.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
EPHB4-202 ENST00000360620.7 3789 935aa Protein coding - Q96L35 - TSL:1, GENCODE basic,
EPHB4-210 ENST00000616502.4 3737 306aa Protein coding - P54760-3 - TSL:5, GENCODE basic,
EPHB4-204 ENST00000477446.5 1551 No protein Processed transcript - - - TSL:1,
EPHB4-207 ENST00000489808.1 921 No protein Processed transcript - - - TSL:1,
EPHB4-206 ENST00000487222.5 5058 No protein Retained intron - - - TSL:1,
EPHB4-205 ENST00000478459.5 965 No protein Retained intron - - - TSL:2,
EPHB4-208 ENST00000492403.1 812 No protein Retained intron - - - TSL:2,
EPHB4-209 ENST00000492878.1 769 No protein Retained intron - - - TSL:5,
EPHB4-203 ENST00000467515.1 709 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene EPHB4
This KO Strategy loading
Red Cotton™ Notes Gene EPHB4 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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