CRISPR-U™ Gene Knockout Cell Line
Strategy
EPHB4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EPHB4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EPHB4 |
| Gene id | 2050 |
| Organism | Homo sapiens |
| Official full symbol | EPH receptor B4 |
| Gene type | protein-coding |
| Also known as | CMAVM2, HFASD, HTK, LMPHM7, MYK1, TYRO11 |
| Summary | Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EPHB4-201 | ENST00000358173.8 | 4353 | 987aa | Protein coding | CCDS5706 | P54760-1 | NM_004444.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| EPHB4-202 | ENST00000360620.7 | 3789 | 935aa | Protein coding | - | Q96L35 | - | TSL:1, GENCODE basic, |
| EPHB4-210 | ENST00000616502.4 | 3737 | 306aa | Protein coding | - | P54760-3 | - | TSL:5, GENCODE basic, |
| EPHB4-204 | ENST00000477446.5 | 1551 | No protein | Processed transcript | - | - | - | TSL:1, |
| EPHB4-207 | ENST00000489808.1 | 921 | No protein | Processed transcript | - | - | - | TSL:1, |
| EPHB4-206 | ENST00000487222.5 | 5058 | No protein | Retained intron | - | - | - | TSL:1, |
| EPHB4-205 | ENST00000478459.5 | 965 | No protein | Retained intron | - | - | - | TSL:2, |
| EPHB4-208 | ENST00000492403.1 | 812 | No protein | Retained intron | - | - | - | TSL:2, |
| EPHB4-209 | ENST00000492878.1 | 769 | No protein | Retained intron | - | - | - | TSL:5, |
| EPHB4-203 | ENST00000467515.1 | 709 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EPHB4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EPHB4 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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