CRISPR-U™ Gene Knockout Cell Line Strategy

HIPK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HIPK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HIPK1
Gene id 204851
Organism Homo sapiens
Official full symbol homeodomain interacting protein kinase 1
Gene type protein-coding
Also known as Myak, Nbak2
Summary The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms.
Genomic regions Chromosome 1
Strategy Summary
This gene has 11 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HIPK1-208 ENST00000426820.7 8201 1210aa Protein coding CCDS867 Q86Z02-1 NM_198268.3 TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92,
HIPK1-205 ENST00000369558.5 8157 1210aa Protein coding CCDS867 Q86Z02-1 - TSL:1, GENCODE basic, APPRIS P1,
HIPK1-201 ENST00000340480.8 6997 836aa Protein coding CCDS41370 Q86Z02-3 - TSL:1, GENCODE basic,
HIPK1-206 ENST00000369559.8 3424 1075aa Protein coding CCDS868 Q86Z02-2 - TSL:1, GENCODE basic,
HIPK1-203 ENST00000369553.5 2883 816aa Protein coding CCDS869 Q86Z02-4 - TSL:1, GENCODE basic,
HIPK1-211 ENST00000626993.2 8016 1165aa Protein coding - Q5SQL3 - TSL:5, GENCODE basic,
HIPK1-202 ENST00000361587.3 5766 491aa Protein coding - H3BLW0 - CDS 5' incomplete, TSL:1,
HIPK1-204 ENST00000369555.6 3966 1165aa Protein coding - Q5SQL3 - TSL:5, GENCODE basic,
HIPK1-207 ENST00000369561.8 3777 1176aa Protein coding - J3KP92 - TSL:5, GENCODE basic,
HIPK1-210 ENST00000514621.5 574 119aa Protein coding - D6RC95 - CDS 3' incomplete, TSL:4,
HIPK1-209 ENST00000503968.1 570 106aa Protein coding - D6RF28 - CDS 3' incomplete, TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HIPK1
This KO Strategy loading
Red Cotton™ Notes Gene HIPK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×

Comment: