CRISPR-U™ Gene Knockout Cell Line
Strategy
HIPK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HIPK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HIPK1 |
| Gene id | 204851 |
| Organism | Homo sapiens |
| Official full symbol | homeodomain interacting protein kinase 1 |
| Gene type | protein-coding |
| Also known as | Myak, Nbak2 |
| Summary | The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HIPK1-208 | ENST00000426820.7 | 8201 | 1210aa | Protein coding | CCDS867 | Q86Z02-1 | NM_198268.3 | TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HIPK1-205 | ENST00000369558.5 | 8157 | 1210aa | Protein coding | CCDS867 | Q86Z02-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| HIPK1-201 | ENST00000340480.8 | 6997 | 836aa | Protein coding | CCDS41370 | Q86Z02-3 | - | TSL:1, GENCODE basic, |
| HIPK1-206 | ENST00000369559.8 | 3424 | 1075aa | Protein coding | CCDS868 | Q86Z02-2 | - | TSL:1, GENCODE basic, |
| HIPK1-203 | ENST00000369553.5 | 2883 | 816aa | Protein coding | CCDS869 | Q86Z02-4 | - | TSL:1, GENCODE basic, |
| HIPK1-211 | ENST00000626993.2 | 8016 | 1165aa | Protein coding | - | Q5SQL3 | - | TSL:5, GENCODE basic, |
| HIPK1-202 | ENST00000361587.3 | 5766 | 491aa | Protein coding | - | H3BLW0 | - | CDS 5' incomplete, TSL:1, |
| HIPK1-204 | ENST00000369555.6 | 3966 | 1165aa | Protein coding | - | Q5SQL3 | - | TSL:5, GENCODE basic, |
| HIPK1-207 | ENST00000369561.8 | 3777 | 1176aa | Protein coding | - | J3KP92 | - | TSL:5, GENCODE basic, |
| HIPK1-210 | ENST00000514621.5 | 574 | 119aa | Protein coding | - | D6RC95 | - | CDS 3' incomplete, TSL:4, |
| HIPK1-209 | ENST00000503968.1 | 570 | 106aa | Protein coding | - | D6RF28 | - | CDS 3' incomplete, TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HIPK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HIPK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: