CRISPR-U™ Gene Knockout Cell Line
Strategy
AK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human AK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | AK1 |
| Gene id | 203 |
| Organism | Homo sapiens |
| Official full symbol | adenylate kinase 1 |
| Gene type | protein-coding |
| Also known as | HTL-S-58j |
| Summary | This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| AK1-206 | ENST00000644144.2 | 2192 | 194aa | Protein coding | CCDS6881 | P00568 Q6FGX9 | NM_000476.3 | GENCODE basic, APPRIS P1, MANE Select v0.92, |
| AK1-202 | ENST00000373156.5 | 757 | 194aa | Protein coding | CCDS6881 | P00568 Q6FGX9 | - | TSL:3, GENCODE basic, APPRIS P1, |
| AK1-201 | ENST00000223836.10 | 736 | 210aa | Protein coding | CCDS83419 | Q5T9B7 | - | TSL:3, GENCODE basic, |
| AK1-205 | ENST00000550992.1 | 574 | 32aa | Nonsense mediated decay | - | F8VRY5 | - | TSL:4, |
| AK1-204 | ENST00000550143.5 | 532 | 51aa | Nonsense mediated decay | - | H0YID2 | - | CDS 5' incomplete, TSL:5, |
| AK1-203 | ENST00000413016.5 | 525 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | AK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene AK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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