CRISPR-U™ Gene Knockout Cell Line Strategy

AK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human AK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol AK1
Gene id 203
Organism Homo sapiens
Official full symbol adenylate kinase 1
Gene type protein-coding
Also known as HTL-S-58j
Summary This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Genomic regions Chromosome 9
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
AK1-206 ENST00000644144.2 2192 194aa Protein coding CCDS6881 P00568 Q6FGX9 NM_000476.3 GENCODE basic, APPRIS P1, MANE Select v0.92,
AK1-202 ENST00000373156.5 757 194aa Protein coding CCDS6881 P00568 Q6FGX9 - TSL:3, GENCODE basic, APPRIS P1,
AK1-201 ENST00000223836.10 736 210aa Protein coding CCDS83419 Q5T9B7 - TSL:3, GENCODE basic,
AK1-205 ENST00000550992.1 574 32aa Nonsense mediated decay - F8VRY5 - TSL:4,
AK1-204 ENST00000550143.5 532 51aa Nonsense mediated decay - H0YID2 - CDS 5' incomplete, TSL:5,
AK1-203 ENST00000413016.5 525 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene AK1
This KO Strategy loading
Red Cotton™ Notes Gene AK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×