CRISPR-U™ Gene Knockout Cell Line Strategy

CTTN Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CTTN Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CTTN
Gene id 2017
Organism Homo sapiens
Official full symbol cortactin
Gene type protein-coding
Also known as EMS1
Summary This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.
Genomic regions Chromosome 11
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CTTN-202 ENST00000346329.7 3272 513aa Protein coding CCDS8197 Q14247-3 - TSL:1, GENCODE basic,
CTTN-201 ENST00000301843.13 3249 550aa Protein coding CCDS41680 Q14247-1 NM_005231.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CTTN-203 ENST00000376561.7 2247 634aa Protein coding CCDS53676 Q14247-2 - TSL:1, GENCODE basic,
CTTN-213 ENST00000529736.5 952 265aa Protein coding - H0YCD9 - CDS 5' incomplete, TSL:3,
CTTN-215 ENST00000533931.1 567 128aa Nonsense mediated decay - H0YEV2 - CDS 5' incomplete, TSL:3,
CTTN-204 ENST00000393747.3 1315 No protein Processed transcript - - - TSL:5,
CTTN-211 ENST00000527622.5 597 No protein Processed transcript - - - TSL:4,
CTTN-210 ENST00000525852.3 1470 No protein Retained intron - - - TSL:5,
CTTN-206 ENST00000482755.6 1087 No protein Retained intron - - - TSL:2,
CTTN-205 ENST00000415461.2 1021 No protein Retained intron - - - TSL:3,
CTTN-207 ENST00000483662.2 736 No protein Retained intron - - - TSL:2,
CTTN-212 ENST00000527962.5 587 No protein Retained intron - - - TSL:2,
CTTN-209 ENST00000525276.1 584 No protein Retained intron - - - TSL:3,
CTTN-208 ENST00000498223.3 523 No protein Retained intron - - - TSL:3,
CTTN-214 ENST00000532334.1 481 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CTTN
This KO Strategy loading
Red Cotton™ Notes Gene CTTN had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×