CRISPR-U™ Gene Knockout Cell Line
Strategy
CTTN Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CTTN Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CTTN |
| Gene id | 2017 |
| Organism | Homo sapiens |
| Official full symbol | cortactin |
| Gene type | protein-coding |
| Also known as | EMS1 |
| Summary | This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CTTN-202 | ENST00000346329.7 | 3272 | 513aa | Protein coding | CCDS8197 | Q14247-3 | - | TSL:1, GENCODE basic, |
| CTTN-201 | ENST00000301843.13 | 3249 | 550aa | Protein coding | CCDS41680 | Q14247-1 | NM_005231.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CTTN-203 | ENST00000376561.7 | 2247 | 634aa | Protein coding | CCDS53676 | Q14247-2 | - | TSL:1, GENCODE basic, |
| CTTN-213 | ENST00000529736.5 | 952 | 265aa | Protein coding | - | H0YCD9 | - | CDS 5' incomplete, TSL:3, |
| CTTN-215 | ENST00000533931.1 | 567 | 128aa | Nonsense mediated decay | - | H0YEV2 | - | CDS 5' incomplete, TSL:3, |
| CTTN-204 | ENST00000393747.3 | 1315 | No protein | Processed transcript | - | - | - | TSL:5, |
| CTTN-211 | ENST00000527622.5 | 597 | No protein | Processed transcript | - | - | - | TSL:4, |
| CTTN-210 | ENST00000525852.3 | 1470 | No protein | Retained intron | - | - | - | TSL:5, |
| CTTN-206 | ENST00000482755.6 | 1087 | No protein | Retained intron | - | - | - | TSL:2, |
| CTTN-205 | ENST00000415461.2 | 1021 | No protein | Retained intron | - | - | - | TSL:3, |
| CTTN-207 | ENST00000483662.2 | 736 | No protein | Retained intron | - | - | - | TSL:2, |
| CTTN-212 | ENST00000527962.5 | 587 | No protein | Retained intron | - | - | - | TSL:2, |
| CTTN-209 | ENST00000525276.1 | 584 | No protein | Retained intron | - | - | - | TSL:3, |
| CTTN-208 | ENST00000498223.3 | 523 | No protein | Retained intron | - | - | - | TSL:3, |
| CTTN-214 | ENST00000532334.1 | 481 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CTTN |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CTTN had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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