CRISPR-U™ Gene Knockout Cell Line
Strategy
EMP1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EMP1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EMP1 |
| Gene id | 2012 |
| Organism | Homo sapiens |
| Official full symbol | epithelial membrane protein 1 |
| Gene type | protein-coding |
| Also known as | CL-20, EMP-1, TMP |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EMP1-201 | ENST00000256951.10 | 5913 | 157aa | Protein coding | CCDS8660 | P54849-1 | NM_001423.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| EMP1-211 | ENST00000544053.5 | 1436 | 94aa | Protein coding | - | F5H2P5 | - | TSL:1, GENCODE basic, |
| EMP1-203 | ENST00000431267.2 | 1039 | 90aa | Protein coding | - | J3KQX4 | - | TSL:1, GENCODE basic, |
| EMP1-202 | ENST00000396301.7 | 675 | 141aa | Protein coding | - | P54849-2 | - | TSL:2, GENCODE basic, |
| EMP1-207 | ENST00000538364.5 | 571 | 146aa | Protein coding | - | F5GYV0 | - | CDS 3' incomplete, TSL:2, |
| EMP1-210 | ENST00000542474.5 | 555 | 3aa | Protein coding | - | - | - | CDS 3' incomplete, TSL:4, |
| EMP1-206 | ENST00000537612.1 | 505 | 69aa | Protein coding | - | F5H397 | - | TSL:3, GENCODE basic, |
| EMP1-209 | ENST00000542289.1 | 579 | No protein | Processed transcript | - | - | - | TSL:4, |
| EMP1-204 | ENST00000535134.5 | 558 | No protein | Processed transcript | - | - | - | TSL:4, |
| EMP1-212 | ENST00000546357.1 | 809 | No protein | Retained intron | - | - | - | TSL:4, |
| EMP1-205 | ENST00000536383.1 | 587 | No protein | Retained intron | - | - | - | TSL:2, |
| EMP1-208 | ENST00000541935.5 | 521 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EMP1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EMP1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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