CRISPR-U™ Gene Knockout Cell Line Strategy

EMP1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human EMP1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol EMP1
Gene id 2012
Organism Homo sapiens
Official full symbol epithelial membrane protein 1
Gene type protein-coding
Also known as CL-20, EMP-1, TMP
Genomic regions Chromosome 12
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
EMP1-201 ENST00000256951.10 5913 157aa Protein coding CCDS8660 P54849-1 NM_001423.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
EMP1-211 ENST00000544053.5 1436 94aa Protein coding - F5H2P5 - TSL:1, GENCODE basic,
EMP1-203 ENST00000431267.2 1039 90aa Protein coding - J3KQX4 - TSL:1, GENCODE basic,
EMP1-202 ENST00000396301.7 675 141aa Protein coding - P54849-2 - TSL:2, GENCODE basic,
EMP1-207 ENST00000538364.5 571 146aa Protein coding - F5GYV0 - CDS 3' incomplete, TSL:2,
EMP1-210 ENST00000542474.5 555 3aa Protein coding - - - CDS 3' incomplete, TSL:4,
EMP1-206 ENST00000537612.1 505 69aa Protein coding - F5H397 - TSL:3, GENCODE basic,
EMP1-209 ENST00000542289.1 579 No protein Processed transcript - - - TSL:4,
EMP1-204 ENST00000535134.5 558 No protein Processed transcript - - - TSL:4,
EMP1-212 ENST00000546357.1 809 No protein Retained intron - - - TSL:4,
EMP1-205 ENST00000536383.1 587 No protein Retained intron - - - TSL:2,
EMP1-208 ENST00000541935.5 521 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene EMP1
This KO Strategy loading
Red Cotton™ Notes Gene EMP1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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