CRISPR-U™ Gene Knockout Cell Line
Strategy
FLCN Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FLCN Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FLCN |
| Gene id | 201163 |
| Organism | Homo sapiens |
| Official full symbol | folliculin |
| Gene type | protein-coding |
| Also known as | BHD, DENND8B, FLCL |
| Summary | This gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene are associated with Birt-Hogg-Dube syndrome, which is characterized by fibrofolliculomas, renal tumors, lung cysts, and pneumothorax. Alternative splicing of this gene results in two transcript variants encoding different isoforms. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FLCN-201 | ENST00000285071.9 | 3667 | 579aa | Protein coding | CCDS32579 | Q8NFG4-1 | NM_144997.7 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| FLCN-203 | ENST00000389169.9 | 1692 | 342aa | Protein coding | CCDS32580 | Q8NFG4-2 | - | TSL:1, GENCODE basic, |
| FLCN-205 | ENST00000417064.1 | 757 | 153aa | Protein coding | - | C9J4C4 | - | CDS 3' incomplete, TSL:2, |
| FLCN-206 | ENST00000461699.1 | 243 | 37aa | Protein coding | - | J3QQZ7 | - | CDS 3' incomplete, TSL:3, |
| FLCN-202 | ENST00000389168.6 | 3359 | No protein | Retained intron | - | - | - | TSL:2, |
| FLCN-207 | ENST00000466317.1 | 2684 | No protein | Retained intron | - | - | - | TSL:2, |
| FLCN-204 | ENST00000389171.4 | 2523 | No protein | Retained intron | - | - | - | TSL:2, |
| FLCN-209 | ENST00000480316.1 | 786 | No protein | Retained intron | - | - | - | TSL:2, |
| FLCN-208 | ENST00000473853.1 | 384 | No protein | Retained intron | - | - | - | TSL:5, |
| FLCN-210 | ENST00000577591.1 | 296 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FLCN |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FLCN had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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