CRISPR-U™ Gene Knockout Cell Line Strategy

FLCN Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FLCN Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FLCN
Gene id 201163
Organism Homo sapiens
Official full symbol folliculin
Gene type protein-coding
Also known as BHD, DENND8B, FLCL
Summary This gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene are associated with Birt-Hogg-Dube syndrome, which is characterized by fibrofolliculomas, renal tumors, lung cysts, and pneumothorax. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Genomic regions Chromosome 17
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FLCN-201 ENST00000285071.9 3667 579aa Protein coding CCDS32579 Q8NFG4-1 NM_144997.7 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
FLCN-203 ENST00000389169.9 1692 342aa Protein coding CCDS32580 Q8NFG4-2 - TSL:1, GENCODE basic,
FLCN-205 ENST00000417064.1 757 153aa Protein coding - C9J4C4 - CDS 3' incomplete, TSL:2,
FLCN-206 ENST00000461699.1 243 37aa Protein coding - J3QQZ7 - CDS 3' incomplete, TSL:3,
FLCN-202 ENST00000389168.6 3359 No protein Retained intron - - - TSL:2,
FLCN-207 ENST00000466317.1 2684 No protein Retained intron - - - TSL:2,
FLCN-204 ENST00000389171.4 2523 No protein Retained intron - - - TSL:2,
FLCN-209 ENST00000480316.1 786 No protein Retained intron - - - TSL:2,
FLCN-208 ENST00000473853.1 384 No protein Retained intron - - - TSL:5,
FLCN-210 ENST00000577591.1 296 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FLCN
This KO Strategy loading
Red Cotton™ Notes Gene FLCN had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×

Comment: