CRISPR-U™ Gene Knockout Cell Line Strategy

HTR3D Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HTR3D Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HTR3D
Gene id 200909
Organism Homo sapiens
Official full symbol 5-hydroxytryptamine receptor 3D
Gene type protein-coding
Also known as 5HT3D
Summary The protein encoded this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit D of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a mitogen and a hormone. This hormone has been linked to neuropsychiatric disorders, including anxiety, depression, and migraine. Serotonin receptors causes fast and depolarizing responses in neurons following activation. The genes encoding subunits C, D and E of this type 3 receptor form a cluster on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 3
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HTR3D-202 ENST00000382489.3 1674 454aa Protein coding CCDS54685 Q70Z44-1 - TSL:1, GENCODE basic, APPRIS P1,
HTR3D-203 ENST00000428798.7 1609 404aa Protein coding CCDS46966 Q70Z44-4 NM_001145143.1 TSL:5, GENCODE basic, MANE Select v0.92,
HTR3D-201 ENST00000334128.6 1459 279aa Protein coding CCDS3249 F6WC43 - TSL:1, GENCODE basic,
HTR3D-204 ENST00000453435.1 1043 233aa Protein coding - Q70Z44-3 - TSL:1, GENCODE basic,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HTR3D
This KO Strategy loading
Red Cotton™ Notes Gene HTR3D had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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