CRISPR-U™ Gene Knockout Cell Line
Strategy
HTR3D Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HTR3D Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HTR3D |
| Gene id | 200909 |
| Organism | Homo sapiens |
| Official full symbol | 5-hydroxytryptamine receptor 3D |
| Gene type | protein-coding |
| Also known as | 5HT3D |
| Summary | The protein encoded this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit D of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a mitogen and a hormone. This hormone has been linked to neuropsychiatric disorders, including anxiety, depression, and migraine. Serotonin receptors causes fast and depolarizing responses in neurons following activation. The genes encoding subunits C, D and E of this type 3 receptor form a cluster on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HTR3D-202 | ENST00000382489.3 | 1674 | 454aa | Protein coding | CCDS54685 | Q70Z44-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| HTR3D-203 | ENST00000428798.7 | 1609 | 404aa | Protein coding | CCDS46966 | Q70Z44-4 | NM_001145143.1 | TSL:5, GENCODE basic, MANE Select v0.92, |
| HTR3D-201 | ENST00000334128.6 | 1459 | 279aa | Protein coding | CCDS3249 | F6WC43 | - | TSL:1, GENCODE basic, |
| HTR3D-204 | ENST00000453435.1 | 1043 | 233aa | Protein coding | - | Q70Z44-3 | - | TSL:1, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HTR3D |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HTR3D had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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$49
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$599
Single gRNA lentivirus
Work flow
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