CRISPR-U™ Gene Knockout Cell Line Strategy

ARL13B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ARL13B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ARL13B
Gene id 200894
Organism Homo sapiens
Official full symbol ADP ribosylation factor like GTPase 13B
Gene type protein-coding
Also known as ARL2L1, JBTS8
Summary This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in this gene are the cause of Joubert syndrome 8. Alternate splicing results in multiple transcript variants.
Genomic regions Chromosome 3
Strategy Summary
This gene has 13 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ARL13B-203 ENST00000394222.8 3971 428aa Protein coding CCDS2925 Q3SXY8-1 NM_001174150.2 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
ARL13B-210 ENST00000535334.5 3906 325aa Protein coding CCDS54615 Q3SXY8-3 - TSL:1, GENCODE basic,
ARL13B-201 ENST00000303097.11 3264 321aa Protein coding CCDS2924 Q3SXY8-2 - TSL:1, GENCODE basic,
ARL13B-205 ENST00000471138.5 2206 428aa Protein coding CCDS2925 Q3SXY8-1 - TSL:1, GENCODE basic, APPRIS P2,
ARL13B-211 ENST00000679404.1 3787 403aa Protein coding - - - GENCODE basic,
ARL13B-219 ENST00000679872.1 3717 411aa Protein coding - - - GENCODE basic,
ARL13B-226 ENST00000681380.1 3524 457aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ARL13B-217 ENST00000679666.1 3455 304aa Protein coding - - - GENCODE basic,
ARL13B-227 ENST00000681655.1 3454 403aa Protein coding - - - GENCODE basic,
ARL13B-214 ENST00000679607.1 2980 149aa Protein coding - B4DRI8 - GENCODE basic,
ARL13B-212 ENST00000679587.1 1654 405aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ARL13B-208 ENST00000486562.2 1434 298aa Protein coding - - - TSL:3, GENCODE basic,
ARL13B-216 ENST00000679657.1 961 76aa Protein coding - - - GENCODE basic,
ARL13B-202 ENST00000335438.7 3659 89aa Nonsense mediated decay - F8W837 - TSL:1,
ARL13B-213 ENST00000679601.1 3564 94aa Nonsense mediated decay - - - -
ARL13B-221 ENST00000680430.1 3521 103aa Nonsense mediated decay - - - -
ARL13B-220 ENST00000680414.1 3468 94aa Nonsense mediated decay - - - -
ARL13B-223 ENST00000681013.1 3446 161aa Nonsense mediated decay - - - -
ARL13B-215 ENST00000679654.1 3349 87aa Nonsense mediated decay - - - -
ARL13B-218 ENST00000679739.1 3341 58aa Nonsense mediated decay - - - -
ARL13B-204 ENST00000460371.5 3203 56aa Nonsense mediated decay - F8WAY0 - TSL:1,
ARL13B-224 ENST00000681247.1 3127 54aa Nonsense mediated decay - - - -
ARL13B-222 ENST00000680994.1 3267 No protein Processed transcript - - - -
ARL13B-207 ENST00000478400.3 574 No protein Processed transcript - - - TSL:3,
ARL13B-225 ENST00000681377.1 3489 No protein Retained intron - - - -
ARL13B-206 ENST00000475206.3 1735 No protein Retained intron - - - TSL:3,
ARL13B-209 ENST00000492165.3 1399 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ARL13B
This KO Strategy loading
Red Cotton™ Notes Gene ARL13B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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