CRISPR-U™ Gene Knockout Cell Line
Strategy
ARL13B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ARL13B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ARL13B |
| Gene id | 200894 |
| Organism | Homo sapiens |
| Official full symbol | ADP ribosylation factor like GTPase 13B |
| Gene type | protein-coding |
| Also known as | ARL2L1, JBTS8 |
| Summary | This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in this gene are the cause of Joubert syndrome 8. Alternate splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 13 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ARL13B-203 | ENST00000394222.8 | 3971 | 428aa | Protein coding | CCDS2925 | Q3SXY8-1 | NM_001174150.2 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| ARL13B-210 | ENST00000535334.5 | 3906 | 325aa | Protein coding | CCDS54615 | Q3SXY8-3 | - | TSL:1, GENCODE basic, |
| ARL13B-201 | ENST00000303097.11 | 3264 | 321aa | Protein coding | CCDS2924 | Q3SXY8-2 | - | TSL:1, GENCODE basic, |
| ARL13B-205 | ENST00000471138.5 | 2206 | 428aa | Protein coding | CCDS2925 | Q3SXY8-1 | - | TSL:1, GENCODE basic, APPRIS P2, |
| ARL13B-211 | ENST00000679404.1 | 3787 | 403aa | Protein coding | - | - | - | GENCODE basic, |
| ARL13B-219 | ENST00000679872.1 | 3717 | 411aa | Protein coding | - | - | - | GENCODE basic, |
| ARL13B-226 | ENST00000681380.1 | 3524 | 457aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ARL13B-217 | ENST00000679666.1 | 3455 | 304aa | Protein coding | - | - | - | GENCODE basic, |
| ARL13B-227 | ENST00000681655.1 | 3454 | 403aa | Protein coding | - | - | - | GENCODE basic, |
| ARL13B-214 | ENST00000679607.1 | 2980 | 149aa | Protein coding | - | B4DRI8 | - | GENCODE basic, |
| ARL13B-212 | ENST00000679587.1 | 1654 | 405aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ARL13B-208 | ENST00000486562.2 | 1434 | 298aa | Protein coding | - | - | - | TSL:3, GENCODE basic, |
| ARL13B-216 | ENST00000679657.1 | 961 | 76aa | Protein coding | - | - | - | GENCODE basic, |
| ARL13B-202 | ENST00000335438.7 | 3659 | 89aa | Nonsense mediated decay | - | F8W837 | - | TSL:1, |
| ARL13B-213 | ENST00000679601.1 | 3564 | 94aa | Nonsense mediated decay | - | - | - | - |
| ARL13B-221 | ENST00000680430.1 | 3521 | 103aa | Nonsense mediated decay | - | - | - | - |
| ARL13B-220 | ENST00000680414.1 | 3468 | 94aa | Nonsense mediated decay | - | - | - | - |
| ARL13B-223 | ENST00000681013.1 | 3446 | 161aa | Nonsense mediated decay | - | - | - | - |
| ARL13B-215 | ENST00000679654.1 | 3349 | 87aa | Nonsense mediated decay | - | - | - | - |
| ARL13B-218 | ENST00000679739.1 | 3341 | 58aa | Nonsense mediated decay | - | - | - | - |
| ARL13B-204 | ENST00000460371.5 | 3203 | 56aa | Nonsense mediated decay | - | F8WAY0 | - | TSL:1, |
| ARL13B-224 | ENST00000681247.1 | 3127 | 54aa | Nonsense mediated decay | - | - | - | - |
| ARL13B-222 | ENST00000680994.1 | 3267 | No protein | Processed transcript | - | - | - | - |
| ARL13B-207 | ENST00000478400.3 | 574 | No protein | Processed transcript | - | - | - | TSL:3, |
| ARL13B-225 | ENST00000681377.1 | 3489 | No protein | Retained intron | - | - | - | - |
| ARL13B-206 | ENST00000475206.3 | 1735 | No protein | Retained intron | - | - | - | TSL:3, |
| ARL13B-209 | ENST00000492165.3 | 1399 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ARL13B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ARL13B had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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