CRISPR-U™ Gene Knockout Cell Line Strategy

ELK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ELK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ELK1
Gene id 2002
Organism Homo sapiens
Official full symbol ETS transcription factor ELK1
Gene type protein-coding
Summary This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14.
Genomic regions Chromosome X
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ELK1-203 ENST00000376983.8 2876 428aa Protein coding CCDS14283 P19419-1 - TSL:1, GENCODE basic, APPRIS P2,
ELK1-201 ENST00000247161.7 2695 428aa Protein coding CCDS14283 P19419-1 - TSL:1, GENCODE basic, APPRIS P2,
ELK1-202 ENST00000343894.8 736 95aa Protein coding CCDS59165 P19419-2 - TSL:1, GENCODE basic, APPRIS ALT2,
ELK1-204 ENST00000468956.1 769 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ELK1
This KO Strategy loading
Red Cotton™ Notes Gene ELK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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