CRISPR-U™ Gene Knockout Cell Line
Strategy
ELK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ELK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ELK1 |
| Gene id | 2002 |
| Organism | Homo sapiens |
| Official full symbol | ETS transcription factor ELK1 |
| Gene type | protein-coding |
| Summary | This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ELK1-203 | ENST00000376983.8 | 2876 | 428aa | Protein coding | CCDS14283 | P19419-1 | - | TSL:1, GENCODE basic, APPRIS P2, |
| ELK1-201 | ENST00000247161.7 | 2695 | 428aa | Protein coding | CCDS14283 | P19419-1 | - | TSL:1, GENCODE basic, APPRIS P2, |
| ELK1-202 | ENST00000343894.8 | 736 | 95aa | Protein coding | CCDS59165 | P19419-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ELK1-204 | ENST00000468956.1 | 769 | No protein | Processed transcript | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ELK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ELK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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