CRISPR-U™ Gene Knockout Cell Line
Strategy
MLKL Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MLKL Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MLKL |
| Gene id | 197259 |
| Organism | Homo sapiens |
| Official full symbol | mixed lineage kinase domain like pseudokinase |
| Gene type | protein-coding |
| Also known as | hMLKL |
| Summary | This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MLKL-202 | ENST00000308807.12 | 2477 | 471aa | Protein coding | CCDS32487 | Q8NB16-1 | NM_152649.4 | TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MLKL-201 | ENST00000306247.11 | 1781 | 263aa | Protein coding | CCDS45528 | Q8NB16-2 | - | TSL:1, GENCODE basic, |
| MLKL-205 | ENST00000573267.1 | 1004 | 211aa | Protein coding | - | I3L2T9 | - | CDS 3' incomplete, TSL:5, |
| MLKL-206 | ENST00000575686.1 | 567 | 62aa | Protein coding | - | I3L4Z5 | - | CDS 3' incomplete, TSL:4, |
| MLKL-208 | ENST00000576529.1 | 458 | 102aa | Protein coding | - | I3L2U3 | - | CDS 5' incomplete, TSL:5, |
| MLKL-204 | ENST00000571303.1 | 781 | 175aa | Nonsense mediated decay | - | I3L4A6 | - | CDS 5' incomplete, TSL:3, |
| MLKL-203 | ENST00000570846.1 | 3882 | No protein | Retained intron | - | - | - | TSL:2, |
| MLKL-207 | ENST00000575695.5 | 2566 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MLKL |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MLKL had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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