CRISPR-U™ Gene Knockout Cell Line Strategy

MLKL Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MLKL Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MLKL
Gene id 197259
Organism Homo sapiens
Official full symbol mixed lineage kinase domain like pseudokinase
Gene type protein-coding
Also known as hMLKL
Summary This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.
Genomic regions Chromosome 16
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MLKL-202 ENST00000308807.12 2477 471aa Protein coding CCDS32487 Q8NB16-1 NM_152649.4 TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92,
MLKL-201 ENST00000306247.11 1781 263aa Protein coding CCDS45528 Q8NB16-2 - TSL:1, GENCODE basic,
MLKL-205 ENST00000573267.1 1004 211aa Protein coding - I3L2T9 - CDS 3' incomplete, TSL:5,
MLKL-206 ENST00000575686.1 567 62aa Protein coding - I3L4Z5 - CDS 3' incomplete, TSL:4,
MLKL-208 ENST00000576529.1 458 102aa Protein coding - I3L2U3 - CDS 5' incomplete, TSL:5,
MLKL-204 ENST00000571303.1 781 175aa Nonsense mediated decay - I3L4A6 - CDS 5' incomplete, TSL:3,
MLKL-203 ENST00000570846.1 3882 No protein Retained intron - - - TSL:2,
MLKL-207 ENST00000575695.5 2566 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MLKL
This KO Strategy loading
Red Cotton™ Notes Gene MLKL had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×