CRISPR-U™ Gene Knockout Cell Line
Strategy
EPHA2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EPHA2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EPHA2 |
| Gene id | 1969 |
| Organism | Homo sapiens |
| Official full symbol | EPH receptor A2 |
| Gene type | protein-coding |
| Also known as | ARCC2, CTPA, CTPP1, CTRCT6, ECK |
| Summary | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EPHA2-201 | ENST00000358432.8 | 3946 | 976aa | Protein coding | CCDS169 | P29317-1 | NM_004431.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| EPHA2-202 | ENST00000461614.1 | 875 | No protein | Processed transcript | - | - | - | TSL:2, |
| EPHA2-204 | ENST00000480202.1 | 795 | No protein | Processed transcript | - | - | - | TSL:5, |
| EPHA2-203 | ENST00000462805.1 | 438 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EPHA2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EPHA2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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