CRISPR-U™ Gene Knockout Cell Line
Strategy
DTX3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DTX3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DTX3 |
| Gene id | 196403 |
| Organism | Homo sapiens |
| Official full symbol | deltex E3 ubiquitin ligase 3 |
| Gene type | protein-coding |
| Also known as | RNF154, deltex3 |
| Summary | DTX3 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 ). |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DTX3-202 | ENST00000548198.5 | 3199 | 347aa | Protein coding | CCDS41800 | Q8N9I9-1 | - | TSL:2, GENCODE basic, APPRIS ALT1, |
| DTX3-204 | ENST00000548804.5 | 2067 | 347aa | Protein coding | CCDS41800 | Q8N9I9-1 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| DTX3-201 | ENST00000337737.8 | 2028 | 347aa | Protein coding | CCDS41800 | Q8N9I9-1 | NM_178502.4 | TSL:2, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| DTX3-208 | ENST00000551632.1 | 1840 | 350aa | Protein coding | CCDS66410 | Q8N9I9-2 | - | TSL:2, GENCODE basic, APPRIS P4, |
| DTX3-203 | ENST00000548478.1 | 2270 | 194aa | Protein coding | - | A0A0C4DGI8 | - | CDS 3' incomplete, TSL:1, |
| DTX3-205 | ENST00000549583.5 | 1331 | 204aa | Protein coding | - | F8VUZ4 | - | CDS 3' incomplete, TSL:1, |
| DTX3-206 | ENST00000550300.1 | 791 | 172aa | Protein coding | - | H0YHF9 | - | CDS 5' incomplete, TSL:2, |
| DTX3-207 | ENST00000550596.5 | 569 | 7aa | Protein coding | - | A0A1Y8EH36 | - | CDS 3' incomplete, TSL:4, |
| DTX3-209 | ENST00000551835.5 | 561 | 48aa | Protein coding | - | F8VV96 | - | CDS 3' incomplete, TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DTX3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DTX3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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