CRISPR-U™ Gene Knockout Cell Line Strategy

DTX3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DTX3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DTX3
Gene id 196403
Organism Homo sapiens
Official full symbol deltex E3 ubiquitin ligase 3
Gene type protein-coding
Also known as RNF154, deltex3
Summary DTX3 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 ).
Genomic regions Chromosome 12
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DTX3-202 ENST00000548198.5 3199 347aa Protein coding CCDS41800 Q8N9I9-1 - TSL:2, GENCODE basic, APPRIS ALT1,
DTX3-204 ENST00000548804.5 2067 347aa Protein coding CCDS41800 Q8N9I9-1 - TSL:5, GENCODE basic, APPRIS ALT1,
DTX3-201 ENST00000337737.8 2028 347aa Protein coding CCDS41800 Q8N9I9-1 NM_178502.4 TSL:2, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
DTX3-208 ENST00000551632.1 1840 350aa Protein coding CCDS66410 Q8N9I9-2 - TSL:2, GENCODE basic, APPRIS P4,
DTX3-203 ENST00000548478.1 2270 194aa Protein coding - A0A0C4DGI8 - CDS 3' incomplete, TSL:1,
DTX3-205 ENST00000549583.5 1331 204aa Protein coding - F8VUZ4 - CDS 3' incomplete, TSL:1,
DTX3-206 ENST00000550300.1 791 172aa Protein coding - H0YHF9 - CDS 5' incomplete, TSL:2,
DTX3-207 ENST00000550596.5 569 7aa Protein coding - A0A1Y8EH36 - CDS 3' incomplete, TSL:4,
DTX3-209 ENST00000551835.5 561 48aa Protein coding - F8VV96 - CDS 3' incomplete, TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DTX3
This KO Strategy loading
Red Cotton™ Notes Gene DTX3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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