CRISPR-U™ Gene Knockout Cell Line Strategy

EGF Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human EGF Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol EGF
Gene id 1950
Organism Homo sapiens
Official full symbol epidermal growth factor
Gene type protein-coding
Also known as HOMG4, URG
Summary This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Genomic regions Chromosome 4
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
EGF-201 ENST00000265171.10 6388 1207aa Protein coding CCDS3689 P01133-1 NM_001963.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
EGF-206 ENST00000509793.5 4489 1165aa Protein coding CCDS54795 P01133-2 - TSL:2, GENCODE basic,
EGF-204 ENST00000503392.1 3642 1166aa Protein coding CCDS54794 P01133-3 - TSL:1, GENCODE basic,
EGF-213 ENST00000652245.1 5923 1010aa Protein coding - A0A494C018 - GENCODE basic,
EGF-203 ENST00000502723.1 683 No protein Processed transcript - - - TSL:3,
EGF-211 ENST00000541061.1 432 No protein Processed transcript - - - TSL:3,
EGF-207 ENST00000509996.1 1464 No protein Retained intron - - - TSL:1,
EGF-212 ENST00000544918.1 773 No protein Retained intron - - - TSL:3,
EGF-205 ENST00000504633.1 584 No protein Retained intron - - - TSL:3,
EGF-202 ENST00000502579.1 580 No protein Retained intron - - - TSL:3,
EGF-208 ENST00000511228.5 567 No protein Retained intron - - - TSL:4,
EGF-209 ENST00000537316.5 491 No protein Retained intron - - - TSL:3,
EGF-210 ENST00000540840.1 324 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene EGF
This KO Strategy loading
Red Cotton™ Notes Gene EGF had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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