CRISPR-U™ Gene Knockout Cell Line
Strategy
EGF Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EGF Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EGF |
| Gene id | 1950 |
| Organism | Homo sapiens |
| Official full symbol | epidermal growth factor |
| Gene type | protein-coding |
| Also known as | HOMG4, URG |
| Summary | This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EGF-201 | ENST00000265171.10 | 6388 | 1207aa | Protein coding | CCDS3689 | P01133-1 | NM_001963.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| EGF-206 | ENST00000509793.5 | 4489 | 1165aa | Protein coding | CCDS54795 | P01133-2 | - | TSL:2, GENCODE basic, |
| EGF-204 | ENST00000503392.1 | 3642 | 1166aa | Protein coding | CCDS54794 | P01133-3 | - | TSL:1, GENCODE basic, |
| EGF-213 | ENST00000652245.1 | 5923 | 1010aa | Protein coding | - | A0A494C018 | - | GENCODE basic, |
| EGF-203 | ENST00000502723.1 | 683 | No protein | Processed transcript | - | - | - | TSL:3, |
| EGF-211 | ENST00000541061.1 | 432 | No protein | Processed transcript | - | - | - | TSL:3, |
| EGF-207 | ENST00000509996.1 | 1464 | No protein | Retained intron | - | - | - | TSL:1, |
| EGF-212 | ENST00000544918.1 | 773 | No protein | Retained intron | - | - | - | TSL:3, |
| EGF-205 | ENST00000504633.1 | 584 | No protein | Retained intron | - | - | - | TSL:3, |
| EGF-202 | ENST00000502579.1 | 580 | No protein | Retained intron | - | - | - | TSL:3, |
| EGF-208 | ENST00000511228.5 | 567 | No protein | Retained intron | - | - | - | TSL:4, |
| EGF-209 | ENST00000537316.5 | 491 | No protein | Retained intron | - | - | - | TSL:3, |
| EGF-210 | ENST00000540840.1 | 324 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EGF |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EGF had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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