CRISPR-U™ Gene Knockout Cell Line Strategy

Plcb1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human Plcb1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol Plcb1
Gene id 18795
Organism Mus musculus
Official full symbol phospholipase C, beta 1
Gene type protein-coding
Also known as 3110043I21Rik, AI132408, Plcb, mKIAA0581
Genomic regions Chromosome 2
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match Flags
Plcb1-205 ENSMUST00000131552.5 7202 1173aa Protein coding CCDS16787 Q9Z1B3-2 TSL:1, GENCODE basic,
Plcb1-202 ENSMUST00000110116.8 7003 1216aa Protein coding CCDS50729 Q9Z1B3-1 TSL:1, GENCODE basic, APPRIS P1,
Plcb1-201 ENSMUST00000070724.12 3853 1173aa Protein coding CCDS16787 Q9Z1B3-2 TSL:1, GENCODE basic,
Plcb1-206 ENSMUST00000201485.4 626 59aa Nonsense mediated decay - A0A0J9YUY5 CDS 5' incomplete, TSL:3,
Plcb1-207 ENSMUST00000201524.2 774 No protein Processed transcript - - TSL:3,
Plcb1-203 ENSMUST00000129382.5 493 No protein Processed transcript - - TSL:5,
Plcb1-208 ENSMUST00000202531.4 2672 No protein Retained intron - - TSL:1,
Plcb1-204 ENSMUST00000130524.5 1785 No protein Retained intron - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene Plcb1
This KO Strategy loading
Red Cotton™ Notes Gene Plcb1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×