CRISPR-U™ Gene Knockout Cell Line
Strategy
Plcb1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human Plcb1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | Plcb1 |
| Gene id | 18795 |
| Organism | Mus musculus |
| Official full symbol | phospholipase C, beta 1 |
| Gene type | protein-coding |
| Also known as | 3110043I21Rik, AI132408, Plcb, mKIAA0581 |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | Flags |
| Plcb1-205 | ENSMUST00000131552.5 | 7202 | 1173aa | Protein coding | CCDS16787 | Q9Z1B3-2 | TSL:1, GENCODE basic, |
| Plcb1-202 | ENSMUST00000110116.8 | 7003 | 1216aa | Protein coding | CCDS50729 | Q9Z1B3-1 | TSL:1, GENCODE basic, APPRIS P1, |
| Plcb1-201 | ENSMUST00000070724.12 | 3853 | 1173aa | Protein coding | CCDS16787 | Q9Z1B3-2 | TSL:1, GENCODE basic, |
| Plcb1-206 | ENSMUST00000201485.4 | 626 | 59aa | Nonsense mediated decay | - | A0A0J9YUY5 | CDS 5' incomplete, TSL:3, |
| Plcb1-207 | ENSMUST00000201524.2 | 774 | No protein | Processed transcript | - | - | TSL:3, |
| Plcb1-203 | ENSMUST00000129382.5 | 493 | No protein | Processed transcript | - | - | TSL:5, |
| Plcb1-208 | ENSMUST00000202531.4 | 2672 | No protein | Retained intron | - | - | TSL:1, |
| Plcb1-204 | ENSMUST00000130524.5 | 1785 | No protein | Retained intron | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | Plcb1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene Plcb1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: