CRISPR-U™ Gene Knockout Cell Line
Strategy
E2F4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human E2F4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | E2F4 |
| Gene id | 1874 |
| Organism | Homo sapiens |
| Official full symbol | E2F transcription factor 4 |
| Gene type | protein-coding |
| Also known as | E2F-4 |
| Summary | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| E2F4-201 | ENST00000379378.8 | 2110 | 413aa | Protein coding | CCDS32464 | Q16254 | NM_001950.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| E2F4-205 | ENST00000565226.1 | 446 | 144aa | Protein coding | - | I3L1B6 | - | CDS 5' incomplete, TSL:5, |
| E2F4-212 | ENST00000568839.5 | 944 | 145aa | Nonsense mediated decay | - | H3BVE4 | - | TSL:3, |
| E2F4-213 | ENST00000569573.1 | 849 | 121aa | Nonsense mediated decay | - | H3BTM3 | - | TSL:3, |
| E2F4-206 | ENST00000565849.5 | 639 | 81aa | Nonsense mediated decay | - | H3BMP3 | - | TSL:3, |
| E2F4-204 | ENST00000564718.1 | 688 | No protein | Processed transcript | - | - | - | TSL:3, |
| E2F4-208 | ENST00000567007.5 | 2615 | No protein | Retained intron | - | - | - | TSL:2, |
| E2F4-202 | ENST00000561904.5 | 897 | No protein | Retained intron | - | - | - | TSL:3, |
| E2F4-209 | ENST00000567228.1 | 757 | No protein | Retained intron | - | - | - | TSL:2, |
| E2F4-203 | ENST00000563238.5 | 736 | No protein | Retained intron | - | - | - | TSL:2, |
| E2F4-210 | ENST00000568485.1 | 707 | No protein | Retained intron | - | - | - | TSL:2, |
| E2F4-211 | ENST00000568693.1 | 596 | No protein | Retained intron | - | - | - | TSL:2, |
| E2F4-207 | ENST00000566368.1 | 438 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | E2F4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene E2F4 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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