CRISPR-U™ Gene Knockout Cell Line Strategy

E2F4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human E2F4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol E2F4
Gene id 1874
Organism Homo sapiens
Official full symbol E2F transcription factor 4
Gene type protein-coding
Also known as E2F-4
Summary The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
Genomic regions Chromosome 16
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
E2F4-201 ENST00000379378.8 2110 413aa Protein coding CCDS32464 Q16254 NM_001950.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
E2F4-205 ENST00000565226.1 446 144aa Protein coding - I3L1B6 - CDS 5' incomplete, TSL:5,
E2F4-212 ENST00000568839.5 944 145aa Nonsense mediated decay - H3BVE4 - TSL:3,
E2F4-213 ENST00000569573.1 849 121aa Nonsense mediated decay - H3BTM3 - TSL:3,
E2F4-206 ENST00000565849.5 639 81aa Nonsense mediated decay - H3BMP3 - TSL:3,
E2F4-204 ENST00000564718.1 688 No protein Processed transcript - - - TSL:3,
E2F4-208 ENST00000567007.5 2615 No protein Retained intron - - - TSL:2,
E2F4-202 ENST00000561904.5 897 No protein Retained intron - - - TSL:3,
E2F4-209 ENST00000567228.1 757 No protein Retained intron - - - TSL:2,
E2F4-203 ENST00000563238.5 736 No protein Retained intron - - - TSL:2,
E2F4-210 ENST00000568485.1 707 No protein Retained intron - - - TSL:2,
E2F4-211 ENST00000568693.1 596 No protein Retained intron - - - TSL:2,
E2F4-207 ENST00000566368.1 438 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene E2F4
This KO Strategy loading
Red Cotton™ Notes Gene E2F4 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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