CRISPR-U™ Gene Knockout Cell Line
Strategy
E2F3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human E2F3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | E2F3 |
| Gene id | 1871 |
| Organism | Homo sapiens |
| Official full symbol | E2F transcription factor 3 |
| Gene type | protein-coding |
| Also known as | E2F-3 |
| Summary | This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| E2F3-201 | ENST00000346618.8 | 5036 | 465aa | Protein coding | CCDS4545 | O00716-1 | NM_001949.5 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| E2F3-202 | ENST00000535432.2 | 4425 | 334aa | Protein coding | CCDS58999 | O00716-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| E2F3-203 | ENST00000613242.4 | 4094 | 128aa | Protein coding | - | A0A087X0B6 | - | TSL:5, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | E2F3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene E2F3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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