CRISPR-U™ Gene Knockout Cell Line
Strategy
APLNR Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human APLNR Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | APLNR |
| Gene id | 187 |
| Organism | Homo sapiens |
| Official full symbol | apelin receptor |
| Gene type | protein-coding |
| Also known as | AGTRL1, APJ, APJR, HG11 |
| Summary | This gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| APLNR-202 | ENST00000606794.1 | 3609 | 380aa | Protein coding | CCDS7950 | P35414 | - | TSL:NA, GENCODE basic, APPRIS P1, |
| APLNR-201 | ENST00000257254.3 | 1726 | 380aa | Nonsense mediated decay | CCDS7950 | P35414 | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | APLNR |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene APLNR had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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