CRISPR-U™ Gene Knockout Cell Line Strategy

APLNR Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human APLNR Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol APLNR
Gene id 187
Organism Homo sapiens
Official full symbol apelin receptor
Gene type protein-coding
Also known as AGTRL1, APJ, APJR, HG11
Summary This gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified.
Genomic regions Chromosome 11
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
APLNR-202 ENST00000606794.1 3609 380aa Protein coding CCDS7950 P35414 - TSL:NA, GENCODE basic, APPRIS P1,
APLNR-201 ENST00000257254.3 1726 380aa Nonsense mediated decay CCDS7950 P35414 - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene APLNR
This KO Strategy loading
Red Cotton™ Notes Gene APLNR had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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