CRISPR-U™ Gene Knockout Cell Line
Strategy
DSG2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DSG2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DSG2 |
| Gene id | 1829 |
| Organism | Homo sapiens |
| Official full symbol | desmoglein 2 |
| Gene type | protein-coding |
| Also known as | CDHF5, HDGC |
| Summary | This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. |
| Genomic regions | Chromosome 18 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DSG2-201 | ENST00000261590.13 | 5697 | 1118aa | Protein coding | CCDS42423 | Q14126 | NM_001943.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DSG2-202 | ENST00000585206.1 | 1081 | 232aa | Protein coding | - | J3KSI6 | - | TSL:2, GENCODE basic, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DSG2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DSG2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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