CRISPR-U™ Gene Knockout Cell Line
Strategy
JAG1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human JAG1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | JAG1 |
| Gene id | 182 |
| Organism | Homo sapiens |
| Official full symbol | jagged canonical Notch ligand 1 |
| Gene type | protein-coding |
| Also known as | AGS, AGS1, AHD, AWS, CD339, DCHE, HJ1, JAGL1 |
| Summary | The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter is involved in signaling processes. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| JAG1-201 | ENST00000254958.10 | 5940 | 1218aa | Protein coding | CCDS13112 | P78504-1 | NM_000214.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| JAG1-205 | ENST00000613518.1 | 597 | 172aa | Protein coding | - | A0A087WXH5 | - | CDS 5' incomplete, TSL:5, |
| JAG1-209 | ENST00000622545.1 | 507 | 74aa | Nonsense mediated decay | - | A0A087X1E8 | - | CDS 5' incomplete, TSL:5, |
| JAG1-202 | ENST00000423891.6 | 3758 | No protein | Processed transcript | - | - | - | TSL:2, |
| JAG1-207 | ENST00000617965.2 | 3385 | No protein | Retained intron | - | - | - | TSL:5, |
| JAG1-203 | ENST00000488480.2 | 1161 | No protein | Retained intron | - | - | - | TSL:4, |
| JAG1-208 | ENST00000620743.1 | 756 | No protein | Retained intron | - | - | - | TSL:3, |
| JAG1-204 | ENST00000612857.1 | 586 | No protein | Retained intron | - | - | - | TSL:5, |
| JAG1-206 | ENST00000617357.1 | 580 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | JAG1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene JAG1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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