CRISPR-U™ Gene Knockout Cell Line Strategy

JAG1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human JAG1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol JAG1
Gene id 182
Organism Homo sapiens
Official full symbol jagged canonical Notch ligand 1
Gene type protein-coding
Also known as AGS, AGS1, AHD, AWS, CD339, DCHE, HJ1, JAGL1
Summary The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter is involved in signaling processes. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.
Genomic regions Chromosome 20
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
JAG1-201 ENST00000254958.10 5940 1218aa Protein coding CCDS13112 P78504-1 NM_000214.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
JAG1-205 ENST00000613518.1 597 172aa Protein coding - A0A087WXH5 - CDS 5' incomplete, TSL:5,
JAG1-209 ENST00000622545.1 507 74aa Nonsense mediated decay - A0A087X1E8 - CDS 5' incomplete, TSL:5,
JAG1-202 ENST00000423891.6 3758 No protein Processed transcript - - - TSL:2,
JAG1-207 ENST00000617965.2 3385 No protein Retained intron - - - TSL:5,
JAG1-203 ENST00000488480.2 1161 No protein Retained intron - - - TSL:4,
JAG1-208 ENST00000620743.1 756 No protein Retained intron - - - TSL:3,
JAG1-204 ENST00000612857.1 586 No protein Retained intron - - - TSL:5,
JAG1-206 ENST00000617357.1 580 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene JAG1
This KO Strategy loading
Red Cotton™ Notes Gene JAG1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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