CRISPR-U™ Gene Knockout Cell Line
Strategy
DR1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DR1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DR1 |
| Gene id | 1810 |
| Organism | Homo sapiens |
| Official full symbol | down-regulator of transcription 1 |
| Gene type | protein-coding |
| Also known as | NC2, NC2-BETA, NC2B, NCB2 |
| Summary | This gene encodes a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The encoded protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DR1-202 | ENST00000370272.9 | 10124 | 176aa | Protein coding | CCDS744 | Q01658 Q658N3 | NM_001938.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DR1-201 | ENST00000370267.1 | 1647 | 176aa | Protein coding | CCDS744 | Q01658 Q658N3 | - | TSL:2, GENCODE basic, APPRIS P1, |
| DR1-203 | ENST00000481583.1 | 1244 | No protein | Processed transcript | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DR1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DR1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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