CRISPR-U™ Gene Knockout Cell Line Strategy

DPP4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DPP4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DPP4
Gene id 1803
Organism Homo sapiens
Official full symbol dipeptidyl peptidase 4
Gene type protein-coding
Also known as ADABP, ADCP2, CD26, DPPIV, TP103
Summary The DPP4 gene encodes dipeptidyl peptidase 4, which is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic type II transmembrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Dipeptidyl peptidase 4 is highly involved in glucose and insulin metabolism, as well as in immune regulation. This protein was shown to be a functional receptor for Middle East respiratory syndrome coronavirus (MERS-CoV), and protein modeling suggests that it may play a similar role with SARS-CoV-2, the virus responsible for COVID-19.
Genomic regions Chromosome 2
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DPP4-201 ENST00000360534.8 3573 766aa Protein coding CCDS2216 P27487 NM_001935.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
DPP4-214 ENST00000676810.1 3749 765aa Protein coding - - - GENCODE basic, APPRIS ALT2,
DPP4-213 ENST00000676768.1 3623 734aa Protein coding - - - GENCODE basic,
DPP4-221 ENST00000678668.1 3339 474aa Protein coding - - - GENCODE basic,
DPP4-219 ENST00000678566.1 3804 296aa Nonsense mediated decay - - - -
DPP4-211 ENST00000676479.1 3551 78aa Nonsense mediated decay - - - -
DPP4-212 ENST00000676624.1 3433 117aa Nonsense mediated decay - - - -
DPP4-223 ENST00000679104.1 3375 118aa Nonsense mediated decay - - - -
DPP4-209 ENST00000494507.2 3045 118aa Nonsense mediated decay - - - TSL:2,
DPP4-203 ENST00000416189.6 2737 118aa Nonsense mediated decay - H7C1N5 - TSL:5,
DPP4-204 ENST00000434918.6 2674 170aa Nonsense mediated decay - F8WE17 - TSL:1,
DPP4-202 ENST00000413651.3 578 118aa Nonsense mediated decay - F8WBB6 - TSL:4,
DPP4-208 ENST00000491591.5 969 No protein Processed transcript - - - TSL:3,
DPP4-206 ENST00000468903.1 582 No protein Processed transcript - - - TSL:4,
DPP4-220 ENST00000678583.1 8539 No protein Retained intron - - - -
DPP4-217 ENST00000677212.1 4977 No protein Retained intron - - - -
DPP4-218 ENST00000678522.1 4796 No protein Retained intron - - - -
DPP4-216 ENST00000677015.1 4574 No protein Retained intron - - - -
DPP4-215 ENST00000676996.1 3433 No protein Retained intron - - - -
DPP4-222 ENST00000678740.1 3391 No protein Retained intron - - - -
DPP4-207 ENST00000490286.5 2241 No protein Retained intron - - - TSL:5,
DPP4-205 ENST00000461836.6 1464 No protein Retained intron - - - TSL:1,
DPP4-210 ENST00000497461.5 781 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DPP4
This KO Strategy loading
Red Cotton™ Notes Gene DPP4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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