CRISPR-U™ Gene Knockout Cell Line
Strategy
DPP4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DPP4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DPP4 |
| Gene id | 1803 |
| Organism | Homo sapiens |
| Official full symbol | dipeptidyl peptidase 4 |
| Gene type | protein-coding |
| Also known as | ADABP, ADCP2, CD26, DPPIV, TP103 |
| Summary | The DPP4 gene encodes dipeptidyl peptidase 4, which is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic type II transmembrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Dipeptidyl peptidase 4 is highly involved in glucose and insulin metabolism, as well as in immune regulation. This protein was shown to be a functional receptor for Middle East respiratory syndrome coronavirus (MERS-CoV), and protein modeling suggests that it may play a similar role with SARS-CoV-2, the virus responsible for COVID-19. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DPP4-201 | ENST00000360534.8 | 3573 | 766aa | Protein coding | CCDS2216 | P27487 | NM_001935.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| DPP4-214 | ENST00000676810.1 | 3749 | 765aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| DPP4-213 | ENST00000676768.1 | 3623 | 734aa | Protein coding | - | - | - | GENCODE basic, |
| DPP4-221 | ENST00000678668.1 | 3339 | 474aa | Protein coding | - | - | - | GENCODE basic, |
| DPP4-219 | ENST00000678566.1 | 3804 | 296aa | Nonsense mediated decay | - | - | - | - |
| DPP4-211 | ENST00000676479.1 | 3551 | 78aa | Nonsense mediated decay | - | - | - | - |
| DPP4-212 | ENST00000676624.1 | 3433 | 117aa | Nonsense mediated decay | - | - | - | - |
| DPP4-223 | ENST00000679104.1 | 3375 | 118aa | Nonsense mediated decay | - | - | - | - |
| DPP4-209 | ENST00000494507.2 | 3045 | 118aa | Nonsense mediated decay | - | - | - | TSL:2, |
| DPP4-203 | ENST00000416189.6 | 2737 | 118aa | Nonsense mediated decay | - | H7C1N5 | - | TSL:5, |
| DPP4-204 | ENST00000434918.6 | 2674 | 170aa | Nonsense mediated decay | - | F8WE17 | - | TSL:1, |
| DPP4-202 | ENST00000413651.3 | 578 | 118aa | Nonsense mediated decay | - | F8WBB6 | - | TSL:4, |
| DPP4-208 | ENST00000491591.5 | 969 | No protein | Processed transcript | - | - | - | TSL:3, |
| DPP4-206 | ENST00000468903.1 | 582 | No protein | Processed transcript | - | - | - | TSL:4, |
| DPP4-220 | ENST00000678583.1 | 8539 | No protein | Retained intron | - | - | - | - |
| DPP4-217 | ENST00000677212.1 | 4977 | No protein | Retained intron | - | - | - | - |
| DPP4-218 | ENST00000678522.1 | 4796 | No protein | Retained intron | - | - | - | - |
| DPP4-216 | ENST00000677015.1 | 4574 | No protein | Retained intron | - | - | - | - |
| DPP4-215 | ENST00000676996.1 | 3433 | No protein | Retained intron | - | - | - | - |
| DPP4-222 | ENST00000678740.1 | 3391 | No protein | Retained intron | - | - | - | - |
| DPP4-207 | ENST00000490286.5 | 2241 | No protein | Retained intron | - | - | - | TSL:5, |
| DPP4-205 | ENST00000461836.6 | 1464 | No protein | Retained intron | - | - | - | TSL:1, |
| DPP4-210 | ENST00000497461.5 | 781 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DPP4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DPP4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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