CRISPR-U™ Gene Knockout Cell Line Strategy

DPH2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DPH2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DPH2
Gene id 1802
Organism Homo sapiens
Official full symbol diphthamide biosynthesis 2
Gene type protein-coding
Also known as DPH2L2
Summary This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DPH2-201 ENST00000255108.8 2466 489aa Protein coding CCDS504 Q9BQC3-1 NM_001384.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DPH2-202 ENST00000396758.6 1738 261aa Protein coding CCDS41314 Q9BQC3-2 - TSL:3, GENCODE basic,
DPH2-203 ENST00000459879.1 1013 262aa Protein coding - H0YCR5 - CDS 5' incomplete, TSL:2,
DPH2-209 ENST00000495421.1 2414 93aa Nonsense mediated decay - E9PIY4 - TSL:2,
DPH2-210 ENST00000524776.5 1596 147aa Nonsense mediated decay - E9PMH7 - TSL:2,
DPH2-205 ENST00000476260.1 857 102aa Nonsense mediated decay - E9PPU3 - TSL:5,
DPH2-208 ENST00000492306.5 851 100aa Nonsense mediated decay - E9PLL2 - TSL:5,
DPH2-211 ENST00000527319.1 643 35aa Nonsense mediated decay - H0YED1 - CDS 5' incomplete, TSL:5,
DPH2-215 ENST00000532140.1 502 52aa Nonsense mediated decay - E9PJH6 - TSL:5,
DPH2-213 ENST00000529729.1 1000 No protein Processed transcript - - - TSL:3,
DPH2-206 ENST00000477294.5 639 No protein Processed transcript - - - TSL:3,
DPH2-204 ENST00000471934.1 593 No protein Processed transcript - - - TSL:3,
DPH2-207 ENST00000490861.5 1072 No protein Retained intron - - - TSL:2,
DPH2-212 ENST00000527567.1 824 No protein Retained intron - - - TSL:2,
DPH2-217 ENST00000534786.1 715 No protein Retained intron - - - TSL:3,
DPH2-214 ENST00000530988.1 590 No protein Retained intron - - - TSL:3,
DPH2-216 ENST00000534655.1 558 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DPH2
This KO Strategy loading
Red Cotton™ Notes Gene DPH2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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