CRISPR-U™ Gene Knockout Cell Line
Strategy
DPH2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DPH2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DPH2 |
| Gene id | 1802 |
| Organism | Homo sapiens |
| Official full symbol | diphthamide biosynthesis 2 |
| Gene type | protein-coding |
| Also known as | DPH2L2 |
| Summary | This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DPH2-201 | ENST00000255108.8 | 2466 | 489aa | Protein coding | CCDS504 | Q9BQC3-1 | NM_001384.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DPH2-202 | ENST00000396758.6 | 1738 | 261aa | Protein coding | CCDS41314 | Q9BQC3-2 | - | TSL:3, GENCODE basic, |
| DPH2-203 | ENST00000459879.1 | 1013 | 262aa | Protein coding | - | H0YCR5 | - | CDS 5' incomplete, TSL:2, |
| DPH2-209 | ENST00000495421.1 | 2414 | 93aa | Nonsense mediated decay | - | E9PIY4 | - | TSL:2, |
| DPH2-210 | ENST00000524776.5 | 1596 | 147aa | Nonsense mediated decay | - | E9PMH7 | - | TSL:2, |
| DPH2-205 | ENST00000476260.1 | 857 | 102aa | Nonsense mediated decay | - | E9PPU3 | - | TSL:5, |
| DPH2-208 | ENST00000492306.5 | 851 | 100aa | Nonsense mediated decay | - | E9PLL2 | - | TSL:5, |
| DPH2-211 | ENST00000527319.1 | 643 | 35aa | Nonsense mediated decay | - | H0YED1 | - | CDS 5' incomplete, TSL:5, |
| DPH2-215 | ENST00000532140.1 | 502 | 52aa | Nonsense mediated decay | - | E9PJH6 | - | TSL:5, |
| DPH2-213 | ENST00000529729.1 | 1000 | No protein | Processed transcript | - | - | - | TSL:3, |
| DPH2-206 | ENST00000477294.5 | 639 | No protein | Processed transcript | - | - | - | TSL:3, |
| DPH2-204 | ENST00000471934.1 | 593 | No protein | Processed transcript | - | - | - | TSL:3, |
| DPH2-207 | ENST00000490861.5 | 1072 | No protein | Retained intron | - | - | - | TSL:2, |
| DPH2-212 | ENST00000527567.1 | 824 | No protein | Retained intron | - | - | - | TSL:2, |
| DPH2-217 | ENST00000534786.1 | 715 | No protein | Retained intron | - | - | - | TSL:3, |
| DPH2-214 | ENST00000530988.1 | 590 | No protein | Retained intron | - | - | - | TSL:3, |
| DPH2-216 | ENST00000534655.1 | 558 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DPH2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DPH2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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