CRISPR-U™ Gene Knockout Cell Line Strategy

DNMT3B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DNMT3B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DNMT3B
Gene id 1789
Organism Homo sapiens
Official full symbol DNA methyltransferase 3 beta
Gene type protein-coding
Also known as ICF, ICF1, M.HsaIIIB
Summary CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Genomic regions Chromosome 20
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DNMT3B-202 ENST00000328111.6 4336 853aa Protein coding CCDS13205 Q9UBC3-1 NM_006892.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
DNMT3B-201 ENST00000201963.3 4255 845aa Protein coding CCDS13204 Q9UBC3-6 - TSL:1, GENCODE basic, APPRIS ALT2,
DNMT3B-204 ENST00000353855.6 4237 833aa Protein coding CCDS13206 Q9UBC3-2 - TSL:5, GENCODE basic, APPRIS ALT2,
DNMT3B-203 ENST00000348286.6 4048 770aa Protein coding CCDS13207 Q9UBC3-3 - TSL:1, GENCODE basic,
DNMT3B-205 ENST00000443239.7 2674 728aa Protein coding CCDS56183 Q9UBC3-8 - TSL:2, GENCODE basic,
DNMT3B-206 ENST00000456297.6 2315 694aa Protein coding CCDS56184 Q9UBC3-7 - TSL:2, GENCODE basic,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DNMT3B
This KO Strategy loading
Red Cotton™ Notes Gene DNMT3B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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